Mightex’s market-leading Polygon pattern illuminator provides precise spatiotemporal control of light with subcellular resolution, making it the perfect illumination tool for life science research.
The Polygon uses digital mirror device (DMD) technology to illuminate multiple regions simultaneously. A DMD is composed of hundreds of thousands of micro-mirrors that can be individually turned on to reflect light onto the sample. Thus, you can control each mirror to control the area(s) of illumination and create any number of different sized patterns. The Polygon DMD device can be mounted into the infinity-path of any microscope.
Mightex offers multiple Polygon models that have been designed with different features to meet the needs of a wide-range of applications that use patterned illumination. Please see below for all available Polygon models.
The Polygon400 DSI-G is a flexible solution for patterned illumination, as this patterned illuminator can be used with any lightsource (400-700nm) that accepts a 3mm core lightguide. Thus, the DSI-G provides future flexibility for different wavelengths and lightsources, depending on your application.
The Polygon400 DSI-DP is a flexible solution for UV patterned illumination, as this patterned illuminator can be used with any lightsource that accepts a 3mm core lightguide. Unlike the DSI-G, the DSI-DP has been optimized for UV illumination (350-700nm) applications such as, uncaging and photopatterning. In addition, the DSI-DP provides high-resolution illumination with a smaller field of view.
The Polygon400 DSI-DL is a flexible solution for large field of view or high-power patterned illumination applications, as this patterned illuminator can be used with any fiber-coupled lightsource (400-700nm), such as high-power lasers. This Polygon400 model has been designed for high-power applications, such as in vivo optogenetics.
Mightex’s PolyScan software platform is bundled with every Polygon to help you execute sophisticated patterned illumination experiments for your research. It provides:
The Polygon enables scientists to precisely control where light will hit their sample. With subcellular resolution, the Polygon can illuminate any cellular feature in any shape or size. Our pattern editor allows the researcher to draw any pattern in true dimension or import an image of interest to project onto their sample, providing full spatial control of illumination.
The Polygon provides great flexibility when it comes down to wavelength selection. From UV to VIS/NIR range, the Polygon can project light of different colours suitable for your light-sensitive constructs.
Unlike a point scanner that sequentially illuminates a region-of-interest pixel by pixel, the Polygon projects all ON pixels simultaneously such that multiple regions-of-interest are illuminated at the exact same time.
Control the initiation, duration, and intensity of light stimulation patterns using the Polygon. Create different waveforms to control the light intensity and duration outputted from the Polygon.
The Polygon400 can switch between patterns at up to 4000Hz. This pattern switching speed is critical for studying physiologically relevant cellular events, such as action potentials.
The Polygon is designed to be coupled into the infinity space of any microscope model (Leica, Nikon, Olympus, Zeiss) with Mightex’s microscope-specific adapters. Please contact Mightex to discuss the different options available for coupling to a microscope.
The Polygon comes with BNC connectorized TTL trigger input and output for easy synchronization with multiple lab equipment. Synchronize the Polygon400 with your motorized stage for field of view stitching, or synchronize your stimulation patterns with electrophysiology equipment.
The Polygon works with any third-party camera. All the Polygon400 needs is access to the imaging acquisition window for calibration and pattern creation.
Mightex offers different microscope adapters for all inverted and upright microscopes. The microscope adapter is designed to couple the Polygon into the infinity-path of the microscope for patterned illumination capabilities.
Mightex’s Multi-Port Illuminator (MPI) enables researchers to add a Polygon Patterned Illuminator to their microscope for targeted illumination while keeping their original light source for epi-fluorescence or other wide-field stimulation. MPI can be used in any inverted or upright microscope (e.g. Leica, Nikon, Olympus and Zeiss).