GCaMP imaging in the striatum of a mouse with a 600um field of view using the OASIS Implant.
GCaMP imaging in the striatum of a mouse with a 300um field of view using the OASIS Implant
Calcium imaging in a freely-behaving mouse with the OASIS Implant.
Simultaneous Imaging and Optogenetics
The OASIS Implant has a reconfigurable platform to be configured for multiple imaging and optogenetic applications. Please see below for an outline of the OASIS Implant components.
The OASIS Implant has two illumination ports for multiple light sources to be inserted. This allows the OASIS Implant to be used for different imaging and stimulation applications.
The OASIS Implant is compatible with any light source (LED, laser, halogen lamp etc.) via lightguide or fiber. Our Polygon400 Patterned Illuminator is compatible with the OASIS Implant to selectively illuminate/stimulate select neurons or areas of interest in vivo.
Each illumination port (2) on the OASIS Implant can hold up to 3 standard filter sets to meet the needs of multiple imaging and stimulation applications.
The OASIS Implant is compatible with any C-Mount camera. An external camera allows the OASIS Implant to be used with high-speed/sensitivity scientific cameras for increased imaging quality for quantitative imaging capture and analysis.
The OASIS Implant uses an imaging fiber (300um or 600um) to transmit and collect light from the deep-brain of a freely-behaving animal for imaging and optogenetics. A standard SMA fiber is compatible with the OASIS Implant for fiber photometry experiments.
The OASIS Implant is designed to be a futureproof platform that can be reconfigured by customers to suit their current and future imaging and optogenetic research on freely behaving animals. Please see below for examples of different OASIS Implant configurations.
If your specific application is not covered by the example configurations below, please contact Mightex directly for technical support.
Widefield imaging of GCaMP or RCaMP labeled-cells in the deep-brain of a freely-behaving animal.
Dual-colour widefield imaging of GCaMP and RCaMP labeled-cells in the deep-brain of a freely-behaving animal.
Simultaneous widefield imaging of GCaMP or RCaMP labeled-cells and widefield red-shifted or blue-shifted optogenetics in the deep-brain of a freely-behaving animal.
Simultaneous widefield imaging of GCaMP or RCaMP labeled-cells and targeted red-shifted or blue-shifted optogenetics in the deep-brain of a freely-behaving animal.
Example of a mouse freely-behaving in it’s homecage with the OASIS Implant attached.
Example of deep-brain imaging of GCaMP6 neurons in the mouse striatum using the OASIS Implant.
Are you interested in all-optical methods for probing in vivo neural activity and deciding what tool is right for you?
This paper will discuss several tools that enable in vivo calcium imaging and optogenetics and their relative merits in an experimental setting. Specifically, the advantages and drawbacks of two-photon imaging, head-mounted microscopes, fiber photometry, and fiberscopes for in vivo imaging and optogenetics will be discussed. Read more.