Optogenetics and Imaging
Illuminate subcellular, single-cell, multiple-cells, or multiple areas with the OASIS Implant. Light of any size and shape can be projected, providing full spatial control of illumination.
Courtesy of Noaf Salah and Dr. Zhigang He (Harvard Medical School)
Discover new calcium signatures with high-quality image acquisition and large data post-acquisition image analysis with the OASIS Implant system.
Courtesy of the NIH
The OASIS Implant reduces the weight of the headmount thereby minimizing animal stress,while at the same time increasing the performance of the microscope.
Courtesy of the NIH
To offer neuroscientists the ability to stimulate and visualize individual neurons in freely-behaving animals, Mightex’s engineers went back to the drawing board and pioneered a new path in the in vivo calcium imaging field. Instead of miniaturizing and placing the microscope on the animal – thereby limiting the microscope’s features and the animal’s behaviour – we kept the microscope out of the animal’s head by making use of long and flexible imaging fibers.
By keeping the microscope away from the animal’s head, the OASIS Implant accomplishes 2 main goals:
Every OASIS Implant system comes with Mightex’s Image Acquisition and Analysis software that allows users to collect large data sets of neural recordings from calcium imaging experiments and provides the key processing and analysis tools required to help users quickly convert these large data sets into results that can be clearly interpreted for analysis.
The software provides a complete set of tools to allow researchers to visualize and capture calcium imaging recordings. The software includes features to synchronize the calcium recordings with the rest of the behavioral equipment and cameras used in the experiment.
Once calcium recordings are acquired, researchers are able to instantly view and process the captured data. The cell identification tools allow for easy detection of cells and sub-cellular features.
Traces of the calcium imaging data can be extracted for each cell that is identified. Along with the cell traces, all inputs and outputs to behavioural equipment/cameras will be contained in collected data sets to allow for easy matching of neural events with behavioral activity.
The OASIS Implant’s unique design provides user with multiple key benefits.
To further dissect neural circuits in detail and discover the contribution of individual neurons to animal behaviour, neuroscientists need a tool to perform cellular-resolution optogenetics in freely- behaving animals.
The OASIS Implant makes use of the capabilities of Mightex’s Polygon400 pattern illuminator to be able to illuminate regions within the field of view, allowing researchers to target individual neurons.
We know neurons send long projections to different parts of the brain to establish connections that are not confined to the same region. Neuroscientists want to better understand the neuronal connections between different regions of the brain.
The OASIS Implant makes use a bifurcated imaging fiber that connects to 2 GRIN lenses, allowing researchers the ability to investigate 2 brain regions simultaneously.
Neuroscientists investigate neuronal populations located at multiple areas of the CNS from surface regions of the brain cortex, all the way down deeper regions, such as the hypothalamus and amygdala, and along the brain stem and spinal cord. Neuroscientists need optical accessibility to these regions, sometimes simultaneously, with minimal disruption.
Neurons in specific brain regions come in distinct types. Some are stimulatory, some are inhibitory, some are GABAergic, some are dopaminergic, etc. Though neuroscientists are able to genetically distinguish these populations, there are only few ways to probe distinct neuronal populations simultaneously.
The OASIS Implant platform allows users to illuminate brain regions with multiple wavelengths simultaneously. Users can transfect multiple neuronal types for investigation of different neuronal circuits within the same brain area. 2 Illumination ports allow the user to combine multiple illumination sources to image or stimulate their neurons of interest. Each illumination port allows up to 3 different filter sets to provide maximum flexibility when combining multiwavelength imaging and targeted stimulation
Biological live imaging can be a messy endeavour. When imaging indicators in vivo, neuroscientists need ways to reliably and confidently collect and analyse the signals obtained, identify individuals cells, and extract high-quality quantitative data such as deltaF/F traces for individual neurons.
Our platform supports the use of high-end cameras that maximize the quality and temporal resolution of the image acquisition.
C-mount Camera Port Allows Cameras With:
– High Signal-to-Noise Ratio
– High Sensitivity
– High Speed
neuroscientists need their animals to be unhindered by the equipment they use to investigate their brains. The ability of the animal to behave as naturally and uninhibited as possible is critical to experimental success.
The OASIS Implant makes use of a small, very light weight head mount (up to 3X lighter compared to other solutions) which allows animals full control of head and body movement. Also, our platform comes with flexible and long imaging fibers that allow animal freedom of movement.
Neuroscientists often plan multiple experiments and are always thinking ahead for the next step in their research.
The OASIS Implant is a modular platform that can be reconfigured to be able to perform different experiments as the need arises. Transition from single to multiwavelength, or from calcium imaging to targeted optogenetics within one single system with the OASIS Implant.
The OASIS Implant is designed to be a highly versatile and fully reconfigurable platform that can satisfy researchers’ current and future optogenetic and imaging research needs on freely-behaving animals. Please see below for examples of different OASIS Implant configurations.
If your specific application is not covered by the example configurations below, please contact Mightex directly for technical support.
Perform simultaneous cellular-resolution optogenetics and calcium imaging in the deep-brain of a freely-behaving animal.
Headmount for Deep Brain Targeted Optogenetics and Calcium Imaging. FoV up to 0.6mm.
Perform simultaneous cellular-resolution optogenetics and calcium imaging in a large cortical region of a freely-behaving animal.
Headmount for Targeted Optogenetics and Calcium Imaging in the Cortex Region. FoV up to 2mm.
Custom FoV supported. Please contact Mightex for details.
Perform simultaneous cellular-resolution optogenetics and calcium imaging in multiple-brain regions of a freely-behaving animal.
The video on the right features the OASIS Implant being used in combination with the Polygon400 to deliver targeted optogenetic stimulation to the cortex of a freely behaving mouse. When the optogenetic stimulation is localized within a specific region in the lower right quadrant the mouse responded with coordinated motor movements resembling a natural behavior. However, when the optogenetic stimulation is localized within a region in the upper right quadrant, the mouse exhibited a different behavior.
Courtesy Prof Zhigang He and Noaf Alwahab, Harvard Medical School
Example of GCaMp6 calcium imaging in the striatum of a freely-behaving mouse with the OASIS Implant.
Are you interested in understanding the link between brain circuits and behaviour?
This paper will discuss the biological and technological challenges to integrating optogenetics into your freely-behaving calcium imaging experiments. Read more.
Are you interested in all-optical methods for probing in vivo neural activity and deciding what tool is right for you?
This paper will discuss several tools that enable in vivo calcium imaging and optogenetics and their relative merits in an experimental setting. Specifically, the advantages and drawbacks of two-photon imaging, head-mounted microscopes, fiber photometry, and fiberscopes for in vivo imaging and optogenetics will be discussed. Read more.