Freely-Behaving Optogenetics and Imaging
System with Single-Cell Resolution
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OASIS Implant
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The OASIS Implant is a ground-breaking platform for single-cell resolution optogenetics and calcium imaging in freely-behaving animals in the deep-braincortex, multiple brain regions, or spinal cord. The OASIS Implant is capable of simultaneous single-cell resolution optogenetics and calcium imaging and is a reconfigurable and scalable platform designed to address both current and future in vivo optogenetics and imaging applications. Truly understand the link between neural circuits and behaviour using the OASIS Implant.

Overview

Manipulate and Map Neural Circuits in Freely-Behaving Animals

Cortex, Deep-Brain, Multi-Region, and Spinal Cord

OASIS Implant

This video shows the OASIS Implant being used in combination with the laser Polygon400 to deliver targeted optogenetic stimulation to different parts of the cortex of a freely-behaving mouse that in turn elicits different behaviours (Courtesy of Dr. Zhigang He and Noaf Alwahab, Harvard Medical School).

Key Product Features

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Typical Applications

Experimental Examples

GCaMP imaging in the striatum of a mouse with a 600um field of view using the OASIS Implant.

This video shows the OASIS Implant being used in combination with the laser Polygon400 to deliver targeted optogenetic stimulation to the cortex of a freely behaving mouse that in turn elicits a specific behaviour (Courtesy of Dr. Zhigang He and Noaf Alwahab, Harvard Medical School).

GCaMP imaging in the striatum of a mouse with a 300um field of view using the OASIS Implant.

Calcium imaging in a freely-behaving mouse with the OASIS Implant.

Technology

Benefits

single-cell optogenetics

Single-Cell Resolution Optogenetics and Calcium Imaging

single-cell resolution

Single-Cell Resolution

reconfigurable

Reconfigurable for Different Experiments

light-weight

Super-Light Head Mount

Components in the OASIS Implant

The OASIS Implant has a reconfigurable, future-proof platform that can be adapted to meet the specific needs of a wide range of imaging and optogenetic applications. Please see below for an outline of the OASIS Implant components.

Multiple Reconfigurable Illumination Ports

The OASIS Implant has two illumination ports, each of which supports multiple light sources with different colors. This enables researchers to adapt the OASIS Implant to suit their specific imaging and optogenetic applications.

Compatible with Any Light Source

The OASIS Implant is compatible with any light source (e.g. LED’s, lasers, and halogen lamps etc.) via a lightguide or a fiber. Mightex’s market-leading Polygon400 Patterned Illuminator is also compatible with the OASIS Implant, enabling researchers to selectively target/stimulate neurons or areas of interest in vivo, both in deep brain and in the cortex region.

Switchable Filter Sets

Each of the two (2) illumination ports on the OASIS Implant contains a filter holder that can hold three (3) filters, and researchers can easily switch between those filters to reconfigure the system to meet their unique imaging and optogenetic research needs.

Compatible with Scientific-Grade Cameras

The OASIS Implant is equipped with a standard C-mount camera port, and this means that the OASIS Implant system can work with any low-noise, high-sensitivity, good-linearity and high-speed scientific camera, enabling high-quality imaging acquisition and high-precision quantitative data analysis. Multiple cameras can also be supported.

Interchangeable Imaging Fiber

The OASIS Implant uses a flexible imaging fiber (300um or 600um) to transmit and collect light from the deep-brain or the cortex of a freely-behaving animal for imaging and optogenetics. A standard SMA fiber is also compatible with the OASIS Implant for fiber photometry experiments.

Configurations

OASIS Implant System Configurations

The OASIS Implant is designed to be a futureproof platform that can be reconfigured by customers to suit their current and future optogenetics and imaging research on freely-behaving animals. Please see below for examples of different OASIS Implant configurations.

If your specific application is not covered by the example configurations below, please contact Mightex directly for technical support.

implant_imaging_targetopto2

Perform simultaneous single-cell resolution optogenetics and calcium imaging in the deep-brain of a freely-behaving animal.

Components: (1) OASIS Implant Platform, (2) Polygon400, (3) Lightguide-Coupled Light Source(s), (4) Light Source Controller(s),  (5) C-Mount Camera, (6) Imaging Fiber, & (7) Headmount + GRIN Lens.

Headmount for Deep Brain Targeted Optogenetics and Calcium Imaging. FoV up to 0.6mm.

implant_imaging_targetopto2_cortex

Perform simultaneous single-cell resolution optogenetics and calcium imaging in a large cortical region of a freely-behaving animal.

Components: (1) OASIS Implant Platform, (2) Polygon400, (3) Any Light Source, (4) Light Source Controller(s),  (5) Any Third-Party Camera, (6) Imaging Fiber, & (7) Headmount.

Headmount for Targeted Optogenetics and Calcium Imaging in the Cortex Region. FoV up to 2mm.

Custom FoV supported. Please contact Mightex for details.

multi_implant_imaging_targetopto2

Perform simultaneous single-cell resolution optogenetics and calcium imaging in multiple-brain regions of a freely-behaving animal.

Components: (1) OASIS Implant Platform, (2) Polygon400, (3) Any Light Source, (4) Light Source Controller(s),  (5) Any Third-Party Camera, (6) Imaging Fiber, & (7) Headmount + GRIN Lens.

Customer Examples

Customer Examples

Cortical Region Optogenetics in Freely-Behaving Mice

The video on the right features the OASIS Implant being used in combination with the Polygon400 to deliver targeted optogenetic stimulation to the cortex of a freely behaving mouse. When the optogenetic stimulation is localized within a specific region in the lower right quadrant the mouse responded with coordinated motor movements resembling a natural behavior. However, when the optogenetic stimulation is localized within a region in the upper right quadrant, the mouse exhibited a different behavior.

Courtesy Prof Zhigang He and Noaf Alwahab, Harvard Medical School

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Deep-Brain Calcium Imaging in a Freely-Behaving Mouse

Example of GCaMp6 calcium imaging in the striatum of a freely-behaving mouse with the OASIS Implant.

Resources

Are you interested in all-optical methods for probing in vivo neural activity and deciding what tool is right for you?

This paper will discuss several tools that enable in vivo calcium imaging and optogenetics and their relative merits in an experimental setting. Specifically, the advantages and drawbacks of two-photon imaging, head-mounted microscopes, fiber photometry, and fiberscopes for in vivo imaging and optogenetics will be discussed. Read more.

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