The OASIS Implant is a ground-breaking platform for simultaneous cellular-resolution optogenetics and calcium imaging in freely-behaving animals to probe complex neuronal networks
To offer neuroscientists the ability to stimulate and visualize individual neurons in freely-behaving animals, Mightex’s engineers went back to the drawing board and pioneered a new path in the in vivo calcium imaging field. Instead of miniaturizing and placing the microscope on the animal – thereby limiting the microscope’s features and the animal’s behaviour – we kept the microscope out of the animal’s head by making use of long and flexible imaging fibers.
By keeping the microscope away from the animal’s head, the OASIS Implant accomplishes 2 main goals:
The OASIS Implant is a modular platform that can be reconfigured to be able to perform different experiments as the need arises. Transition from single- to multi-wavelength experiments, or from calcium imaging to targeted optogenetics within one single system using the OASIS Implant.
Mightex’s OASIS Implant is a ground-breaking microendoscopic system that is capable of all-optical simultaneous imaging and manipulation of neural activity (with single-cell resolution) in the deep brain of freely-behaving animals.
The OASIS Implant with Rotation Adaptive Mechanism (ROAM) includes cutting-edge technology to allow our imaging fibers to rotate while generating minimal torque on the animal and providing the highest degree of animal movement for studies of naturalistic behaviors. When studying behavior paradigms that require constant animal movement and multiple changes of direction, the OASIS Implant ROAM will be the perfect solution.
Every OASIS Implant system comes with Mightex’s Image Acquisition and Analysis software that allows users to collect large data sets of neural recordings from calcium imaging experiments and provides the key processing and analysis tools required to help users quickly convert these large data sets into results that can be clearly interpreted for analysis.
The software provides a complete set of tools to allow researchers to visualize and capture calcium imaging recordings. The software includes features to synchronize the calcium recordings with the rest of the behavioral equipment and cameras used in the experiment.
Once calcium recordings are acquired, researchers are able to instantly view and process the captured data. The cell identification tools allow for easy detection of cells and sub-cellular features.
Traces of the calcium imaging data can be extracted for each cell that is identified. Along with the cell traces, all inputs and outputs to behavioural equipment/cameras will be contained in collected data sets to allow for easy matching of neural events with behavioral activity.
We know neurons send long projections to different parts of the brain to establish connections that are not confined to the same region. Neuroscientists want to better understand the neuronal connections between different regions of the brain.
The OASIS Implant makes use a bifurcated imaging fiber that connects to 2 GRIN lenses, allowing researchers the ability to investigate 2 brain regions simultaneously.
To further dissect neural circuits in detail and discover the contribution of individual neurons to animal behaviour, neuroscientists need a tool to perform cellular-resolution optogenetics in freely-behaving animals.
The OASIS Implant makes use of the capabilities of Mightex’s Polygon pattern illuminator to be able to illuminate regions within the field of view, allowing researchers to target individual neurons.
This video features the OASIS Implant being used in combination with Mightex’s Polygon to deliver targeted optogenetic stimulation to the cortex of a freely behaving mouse. When the optogenetic stimulation is localized within a specific region in the lower right quadrant the mouse responded with coordinated motor movements resembling a natural behavior. However, when the optogenetic stimulation is localized within a region in the upper right quadrant, the mouse exhibits a different behavior.
Biological live imaging can be a messy endeavour. When imaging indicators in vivo, neuroscientists need ways to reliably and confidently collect and analyse the signals obtained. Our platform supports the use of scientific-grade cameras that maximize the quality and temporal resolution of the image acquisition.
C-mount Camera Port Allows Cameras With:
– High Signal-to-Noise Ratio
– High Sensitivity
– High Speed
Neurons in specific brain regions come in distinct types. Some are stimulatory, some are inhibitory, some are GABAergic, some are dopaminergic. Though neuroscientists are able to genetically distinguish these populations, there are only few ways to probe distinct neuronal populations simultaneously.
The OASIS Implant platform allows users to illuminate brain regions with multiple wavelengths simultaneously. Users can transfect multiple neuronal types for investigation of different neuronal circuits within the same brain area.
This headmount is designed for deep-brain applications by coupling the fiber and implanted GRIN lens.
This headmount is designed for large field of view applications (up to 2mm) across the cortex by using a fiber with an increased field of view.