This video shows an OASIS Implant system equipped with Mightex’s laser Polygon400, used to investigate the behavior of a freely-bahaving mouse. The system is capable of delivering precision targeted optogenetic stimulation to different parts of the cortex, and such stimulation in turn elicits different behaviours (Courtesy of Dr. Zhigang He and Noaf Alwahab, Harvard Medical School).
Cellular-Resolution Optogenetics and Calcium Imaging
Reconfigurable for Different Experiments
Super-Light Head Mount
The OASIS Implant is a highly versatile and fully reconfigurable platform that can meet both your current and your future freely-behaving optogenetic and imaging needs with single-neuron resolution. Please see below for an outline of the OASIS Implant components.
The OASIS Implant has two illumination ports, each of which supports multiple light sources with different colors. This enables researchers to adapt the OASIS Implant to suit their specific imaging and optogenetic applications.
The OASIS Implant is compatible with any light source (e.g. LED’s, lasers, and halogen lamps etc.) via a lightguide or a fiber. Mightex’s market-leading Polygon400 Patterned Illuminator is also compatible with the OASIS Implant, enabling researchers to selectively target/stimulate neurons or areas of interest in vivo, both in deep brain and in the cortex region.
Each of the two (2) illumination ports on the OASIS Implant contains a filter holder that can hold three (3) filters, and researchers can easily switch between those filters to reconfigure the system to meet their unique imaging and optogenetic research needs.
The OASIS Implant is equipped with a standard C-mount camera port, and this means that the OASIS Implant system can work with any low-noise, high-sensitivity, good-linearity and high-speed scientific camera, enabling high-quality imaging acquisition and high-precision quantitative data analysis. Multiple cameras can also be supported.
The OASIS Implant uses a flexible imaging fiber (300um or 600um) to transmit and collect light from the deep-brain or the cortex of a freely-behaving animal for imaging and optogenetics. A standard SMA fiber is also compatible with the OASIS Implant for fiber photometry experiments.
The OASIS Implant is designed to be a highly versatile and fully reconfigurable platform that can satisfy researchers’ current and future optogenetic and imaging research needs on freely-behaving animals. Please see below for examples of different OASIS Implant configurations.
If your specific application is not covered by the example configurations below, please contact Mightex directly for technical support.
Perform simultaneous cellular-resolution optogenetics and calcium imaging in the deep-brain of a freely-behaving animal.
Headmount for Deep Brain Targeted Optogenetics and Calcium Imaging. FoV up to 0.6mm.
Perform simultaneous cellular-resolution optogenetics and calcium imaging in a large cortical region of a freely-behaving animal.
Headmount for Targeted Optogenetics and Calcium Imaging in the Cortex Region. FoV up to 2mm.
Custom FoV supported. Please contact Mightex for details.
Perform simultaneous cellular-resolution optogenetics and calcium imaging in multiple-brain regions of a freely-behaving animal.
The video on the right features the OASIS Implant being used in combination with the Polygon400 to deliver targeted optogenetic stimulation to the cortex of a freely behaving mouse. When the optogenetic stimulation is localized within a specific region in the lower right quadrant the mouse responded with coordinated motor movements resembling a natural behavior. However, when the optogenetic stimulation is localized within a region in the upper right quadrant, the mouse exhibited a different behavior.
Courtesy Prof Zhigang He and Noaf Alwahab, Harvard Medical School
Example of GCaMp6 calcium imaging in the striatum of a freely-behaving mouse with the OASIS Implant.
Are you interested in all-optical methods for probing in vivo neural activity and deciding what tool is right for you?
This paper will discuss several tools that enable in vivo calcium imaging and optogenetics and their relative merits in an experimental setting. Specifically, the advantages and drawbacks of two-photon imaging, head-mounted microscopes, fiber photometry, and fiberscopes for in vivo imaging and optogenetics will be discussed. Read more.