Freely-Behaving Cellular-Resolution
Calcium Imaging and Optogenetics
OASIS Implant

Understand the Link Between Neural Circuits and Behaviour

The OASIS Implant is a ground-breaking platform for simultaneous cellular-resolution optogenetics and calcium imaging in freely-behaving animals to probe complex neuronal networks in the deep-brain, cortex, and multiple brain regions, or spinal cord.

  • Freely-Behaving Calcium Imaging and Optogenetics
  • Cellular-Resolution Optogenetics
  • Multi-Region Investigation
  • Reconfigurable Platform
  • High-Quality Imaging with Scientific Cameras

A Different Approach to Integrated Freely-Behaving Calcium Imaging and Optogenetics

To offer neuroscientists the ability to stimulate and visualize individual neurons in freely-behaving animals, Mightex’s engineers went back to the drawing board and pioneered a new path in the in vivo calcium imaging field. Instead of miniaturizing and placing the microscope on the animal – thereby limiting the microscope’s features and the animal’s behaviour – we kept the microscope out of the animal’s head by making use of long and flexible imaging fibers.

How It Works

By keeping the microscope away from the animal’s head, the OASIS Implant accomplishes 2 main goals:

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The Only Reconfigurable Calcium Imaging Platform

The OASIS Implant is a modular platform that can be reconfigured to be able to perform different experiments as the need arises. Transition from single- to multi-wavelength experiments, or from calcium imaging to targeted optogenetics within one single system using the OASIS Implant.

Front View

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Back View

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Models Optimized for Different In Vivo Applications

OASIS Implant

Mightex’s OASIS Implant is a ground-breaking microendoscopic system that is capable of all-optical simultaneous imaging and manipulation of neural activity (with single-cell resolution) in the deep brain of freely-behaving animals.

  • Interchangeable imaging fiber probes to accommodate different imaging/stimulation experiments
  • Furcated fiber probes for multi-region stimulation and imaging
  • Interchangeable cameras, compatible with both GECI and GEVI imaging
  • Reconfigurable platform for calcium imaging and optogenetics
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OASIS Implant ROAM

The OASIS Implant with Rotation Adaptive Mechanism (ROAM) includes cutting-edge technology to allow our imaging fibers to rotate while generating minimal torque on the animal and providing the highest degree of animal movement for studies of naturalistic behaviors. When studying behavior paradigms that require constant animal movement and multiple changes of direction, the OASIS Implant ROAM will be the perfect solution.

  • Bi-directional, 360-degree, unconstrained fiber rotation allowing animals to move and rotate freely
  • Rotation adaptive mechanism for Polygon1000 to precisely track stimulation areas while the animals rotates freely
  • Interchangeable imaging fiber probes to accommodate different imaging/stimulation experiments
  • Furcated fiber probes for multi-region stimulation and imaging
  • Interchangeable cameras, compatible with both GECI and GEVI imaging
  • Motorized stand for easy height adjustment to further enhance freedom of movement
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Intuitive Signal Processing for Data Analysis

Every OASIS Implant system comes with Mightex’s Image Acquisition and Analysis software that allows users to collect large data sets of neural recordings from calcium imaging experiments and provides the key processing and analysis tools required to help users quickly convert these large data sets into results that can be clearly interpreted for analysis.

1. Acquire In Vivo Calcium Recordings

The software provides a complete set of tools to allow researchers to visualize and capture calcium imaging recordings. The software includes features to synchronize the calcium recordings with the rest of the behavioral equipment and cameras used in the experiment.

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2. Identify Cells

Once calcium recordings are acquired, researchers are able to instantly view and process the captured data. The cell identification tools allow for easy detection of cells and sub-cellular features.

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3. Extract DeltaF/F Traces

Traces of the calcium imaging data can be extracted for each cell that is identified. Along with the cell traces, all inputs and outputs to behavioural equipment/cameras will be contained in collected data sets to allow for easy matching of neural events with behavioral activity.

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Access Multiple Brain Regions Simultaneously

We know neurons send long projections to different parts of the brain to establish connections that are not confined to the same region. Neuroscientists want to better understand the neuronal connections between different regions of the brain.

The OASIS Implant makes use a bifurcated imaging fiber that connects to 2 GRIN lenses, allowing researchers the ability to investigate 2 brain regions simultaneously.

Manipulate Functional Neuronal Activity to Control Animal Behaviour

To further dissect neural circuits in detail and discover the contribution of individual neurons to animal behaviour, neuroscientists need a tool to perform cellular-resolution optogenetics in freely-behaving animals.

The OASIS Implant makes use of the capabilities of Mightex’s Polygon pattern illuminator to be able to illuminate regions within the field of view, allowing researchers to target individual neurons.

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Case Study

Optogenetic Stimulation in a Cortical Region

This video features the OASIS Implant being used in combination with Mightex’s Polygon to deliver targeted optogenetic stimulation to the cortex of a freely behaving mouse. When the optogenetic stimulation is localized within a specific region in the lower right quadrant the mouse responded with coordinated motor movements resembling a natural behavior. However, when the optogenetic stimulation is localized within a region in the upper right quadrant, the mouse exhibits a different behavior.

Designed for High-Quality and Flexible Imaging

Collect Weaker GCaMP Signal to Identify More Cells

Biological live imaging can be a messy endeavour. When imaging indicators in vivo, neuroscientists need ways to reliably and confidently collect and analyse the signals obtained. Our platform supports the use of scientific-grade cameras that maximize the quality and temporal resolution of the image acquisition.

C-mount Camera Port Allows Cameras With:
– High Signal-to-Noise Ratio
– High Sensitivity
– High Speed

Investigate Different Neuron Types with Multiwavelength Illumination

Neurons in specific brain regions come in distinct types. Some are stimulatory, some are inhibitory, some are GABAergic, some are dopaminergic. Though neuroscientists are able to genetically distinguish these populations, there are only few ways to probe distinct neuronal populations simultaneously.

The OASIS Implant platform allows users to illuminate brain regions with multiple wavelengths simultaneously. Users can transfect multiple neuronal types for investigation of different neuronal circuits within the same brain area.

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Headmounts Designed for Distinct Applications

Deep-Brain

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This headmount is designed for deep-brain applications by coupling the fiber and implanted GRIN lens.

  • The lightest and smallest head mount in the market.
  • Up to 3X lighter than miniscope alternatives.
  • Provides focusing adjustment and ability to lock both focusing and orientation.

Large Field of View

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This headmount is designed for large field of view applications (up to 2mm) across the cortex by using a fiber with an increased field of view.

  • The lightest and smallest head mount in the market.
  • Up to 3X lighter than miniscope alternatives.
  • Provides focusing adjustment and ability to lock both focusing and orientation.

Worldwide OASIS Implant Customers

Below is a select list of Universities that are using the OASIS Implant.