This video shows our OASIS Macro and laser Polygon400 being used to target ChR2 optogenetic stimulation (200um spots) across the cortex of an anesthetized mouse through a cranial was performed to elicit paw motor movements (Courtesy of Dr. Greg Silasi, University of Ottawa).
Simultaneous Optogenetics and Imaging
Large Field of View
Futureproof with Scalability and Reconfigurability
Versatile Platform with 5 Degrees of Movement
The OASIS Macro is a futureproof scalable and versatile platform which can be reconfigured for different imaging and optogenetic applications. Please see below for an outline of the OASIS Macro components.
The OASIS Macro can be modified to accommodate multiple light sources, depending on the imaging and optogenetic application.
The OASIS Macro is compatible with any light source (LED, laser, halogen lamp etc.) via lightguide or fiber. Our Polygon400 Patterned Illuminator is compatible with the OASIS Macro to selectively illuminate/stimulate areas of interest in vivo.
The OASIS Macro can hold large filter sets to meet the needs of multiple imaging and stimulation applications.
The OASIS Macro can be used with different macro objectives (1X, 1.9X, 2.35X, 4X) that are interchangeable. The appropriate objective will depend on the size of imaging/stimulation area.
Two different stage options are available depending on the animal model and region of interest. The standard translation stage allows for adjustments in the XYZ and can be tilted. The stereotaxic stage allows the objective to be adjusted around the animal from multiple angles to properly control the location of the imaging area.
The OASIS Macro is compatible with any C-Mount camera for large-scale cortical imaging. An external camera allows the OASIS Macro to be used with high-speed/sensitivity scientific cameras for increase imaging quality for quantitative imaging capture and analysis.
The OASIS Macro is designed to be a future-proof platform that can be reconfigured by customers to suit their current or future imaging and optogenetic applications. Please see below for examples of different OASIS Macro configurations.
If your specific application is not covered by the example configurations below, please contact Mightex directly for technical support.
Simultaneous widefield imaging of GCaMP or RCaMP labeled-cells and targeted red-shifted or blue-shifted optogenetics (e.g. Chrimson, JAWS, etc.) in a head-fixed animal.