The OASIS Macro is a mesoscope for all-optical targeted optogenetics, calcium imaging, and intrinsic imaging. The OASIS Macro enables researchers to simultaneously image the entire mouse cortex and perform targeted optogenetics (with Mightex’s market-leading Polygon DMD illuminator).
Mightex’s OASIS Macro is designed as a reconfigurable mesoscope for in vivo imaging and optogenetics. The final system can be tailored to performance requirements for unique applications. This can include major features such as adding a second and third illumination source, or a dual-camera imaging system, as well as fine details including compatibility features for certain cameras and objectives.
The OASIS Macro is designed to image the activity of thousands of neuronal populations across the entire cortex of a head-fixed mouse or large cortical areas of an awake animal. The lenses compatible with the OASIS Macro provide a large field of view, compared to conventional microscopes.
Perform region-specific targeted optogenetics or stimulate the entire cortical area with widefield optogenetics. The addition of Mightex’s Polygon DMD Illuminator to the OASIS Macro enables targeted patterned illumination of regions or cells of interest.
Easily synchronize imaging or optogenetics with other lab equipment. Synchronize with your stimulation patterns or imaging with electrophysiology or behavioural equipment.
The OASIS Macro is designed for in vivo experiments with a minimalist design. A heavy-duty precision XYZ stage is mounted on the support structure and the OASIS optical assembly is in turn mounted on the XYZ stage. The XYZ stage controls the lateral motion as well as the focus of the system.
When imaging indicators in vivo, scientists need ways to reliably and confidently collect and analyse the signals obtained. Our platform supports the use of scientific-grade cameras that maximize the quality and temporal resolution of the image acquisition. Compatible with any C-mount camera or multiple cameras.
Isaac Kauvar and the Deisseroth lab at Stanford used the Polygon DMD illuminator together with Mightex’s large field-of-view OASIS Macro imaging system to simultaneously optogenetically inhibit multiple regions of the mouse’s whole brain cortex. They provide evidence that cortical non-motor areas may play a causal role in motor plan execution.