Cortex-Wide Optogenetics and Imaging System
OASIS Macro
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The OASIS Macro is a ground-breaking macroscope platform for all-optical targeted optogenetics and calcium imaging with a much larger field of view than conventional microscopes, and it enables researchers to simultaneously image the entire mouse cortex and perform targeted optogenetics (with Mightex’s market-leading Polygon400 patterned illuminator). Designed as a scalable, reconfigurable, and versatile in vivo imaging and optogenetic platform, the futureproof design of the OASIS Macro enables researchers to reconfigure the system to fit any new experimental need.

Technology

Benefits

large-scale optogenetics

Simultaneous Optogenetics and Imaging

cortical circuit mapping

Large Field of View

reconfigurable

Futureproof with Scalability and Reconfigurability

rotate

Versatile Platform with 5 Degrees of Movement

Components

The OASIS Macro is a futureproof scalable and versatile platform which can be reconfigured for different imaging and optogenetic applications. Please see below for an outline of the OASIS Macro components.

Multi-Port Illumination

The OASIS Macro can be modified to accommodate multiple light sources, depending on the imaging and optogenetic application.

Light Sources

The OASIS Macro is compatible with any light source (LED, laser, halogen lamp etc.) via lightguide or fiber. Our Polygon400 Patterned Illuminator is compatible with the OASIS Macro to selectively illuminate/stimulate areas of interest in vivo.

Filter Sets

The OASIS Macro can hold large filter sets to meet the needs of multiple imaging and stimulation applications.

Objective

The OASIS Macro can be used with different macro objectives (1X, 1.9X, 2.35X, 4X) that are interchangeable. The appropriate objective will depend on the size of imaging/stimulation area.

Stage

Two different stage options are available depending on the animal model and region of interest. The standard translation stage allows for adjustments in the XYZ and can be tilted. The stereotaxic stage allows the objective to be adjusted around the animal from multiple angles to properly control the location of the imaging area.

External Camera

The OASIS Macro is compatible with any C-Mount camera for large-scale cortical imaging. An external camera allows the OASIS Macro to be used with high-speed/sensitivity scientific cameras for increase imaging quality for quantitative imaging capture and analysis.

Configurations

OASIS Macro System Configuration Examples

The OASIS Macro is designed to be a future-proof platform that can be reconfigured by customers to suit their current or future imaging and optogenetic applications. Please see below for examples of different OASIS Macro configurations.

If your specific application is not covered by the example configurations below, please contact Mightex directly for technical support.

A: Calcium Imaging

Example #1: For Single-Colour Calcium Imaging

Widefield imaging of GCaMP or RCaMP labeled-cells across large cortical areas of a head-fixed animal.

large-scale calcium imaging

Example #2: For Dual-Colour Calcium Imaging

Dual-colour widefield imaging of GCaMP and RCaMP labeled-cells across large cortical areas of a head-fixed animal.

B: Simultaneous Calcium Imaging and Optogenetics

Example #3: For Wide-field Calcium Imaging and Wide-field Optogenetics

Simultaneous wide-field imaging of GCaMP or RCaMP labeled-cells and widefield red-shifted or blue-shifted optogenetics  across large cortical areas of a head-fixed animal.

large-scale calcium imaging and optogenetics

Example #4: For Wide-field Calcium Imaging and Targeted Optogenetics

Simultaneous wide-field imaging of GCaMP or RCaMP labeled-cells, and targeted red-shifted or blue-shifted optogenetics across large cortical areas of a head-fixed animal.

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