In Vivo Neural Circuit Mapping
Optogenetics and Calcium Imaging:
Freely-Behaving
Overview

Optogenetics and calcium imaging have greatly advanced the field of neuroscience by making it possible to manipulate and image neural activity in vivo with single-cell resolution, and applying such capability in a freely-behaving animal enables researchers to stimulate/control and visualize neurons or regions, individually or simultaneously, in order to study the correlation between neural circuits and animal behaviour. Specifically, researchers are able to use the methodology depicted below to investigate neural activity – in a freely behaving animal – in both cortical regions and in the deep brain.

calcium imaging and optogenetcs single-cell
calcium imaging and widefield optogenetics
calcium imaging
cortex or deep-brain

(For more details, click “Deep-Brain” or “Cortex” tabs above)

Deep-Brain

Optogenetics and calcium imaging have greatly advanced the field of neuroscience by making it possible to manipulate and image neural activity in vivo with single-cell resolution, and applying such capability in a freely-behaving animal enables researchers to stimulate/control and visualize neurons, individually or simultaneously, in order to study the correlation between neural circuits and animal behaviour. Specifically, researchers are able to use the methodology depicted below to investigate neural activity – in a freely behaving animal – in the deep brain.

calcium imaging and optogenetcs single-cell
calcium imaging and widefield optogenetics
calcium imaging

Key Requirements

single-cell resolution for imaging and optogenetics
multi-colour stimulate any optogenetic probe
light-weight reduced stress and longer experiments
synchronization

System Configurations

(1) Single-Cell Resolution Optogenetics + Calcium Imaging

implant_imaging_targetopto2

Based on Mightex’s OASIS Implant platform, this configuration provides:

1) Single-cell resolution calcium imaging (GCaMP or RCaMP) in a freely-behaving animal.

2) Single-cell resolution optogenetics (ChR2, Chrimson, NpHr etc.) in a freely-behaving animal using Polygon400.

3) Super light-weight headmount for freely-behaving experiments.

4) Synchronization between imaging and external behavioural equipment.

5) Ability to probe superficial cortical regions and/or deep-brain regions.

Components: (1) OASIS Implant Platform, (2) Polygon400, (3) Any Light Source, (4) Light Source Controller(s),  (5) Any Third-Party Camera, (6) Imaging Fiber, & (7) Headmount + GRIN Lens.

(2) Widefield Optogenetics + Calcium Imaging

implant_imaging_opto2

Based on Mightex’s OASIS Implant platform, this configuration provides:

1) Single-cell resolution calcium imaging (GCaMP or RCaMP) in a freely-behaving animal.

2) Widefield optogenetics (ChR2, Chrimson, NpHr etc.) in a freely-behaving animal.

3) Super light-weight headmount for freely-behaving experiments.

4) Synchronization between imaging and external behavioural equipment.

5) Ability to probe superficial cortical regions and/or deep-brain regions.

Components: (1) OASIS Implant Platform, (2) Any Light Source, (3) Light Source Controller(s),  (4) Any Third-Party Camera, (5) Imaging Fiber, & (6) Headmount + GRIN Lens.

(3) Calcium Imaging

implant_imaging2

Based on Mightex’s OASIS Implant platform, this configuration provides:

1) Single-cell resolution calcium imaging (GCaMP or RCaMP) in a freely-behaving animal.

2) Super light-weight headmount for freely-behaving experiments.

3) Synchronization between imaging and external behavioural equipment.

4) Ability to probe superficial cortical regions and/or deep-brain regions.

Components: (1) OASIS Implant Platform, (2) Any Light Source, (3) Light Source Controller(s),  (4) Any Third-Party Camera, (5) Imaging Fiber, & (6) Headmount + GRIN Lens.

Key Components

(For details, please click on the icons below)

oasis implant freely-behaving calcium imaging and optogenetics
polygon400 single-cell resolution optogenetics

Additional Components

light sources
light source controllers
camera
imaging fiber
Cortex

Optogenetics and calcium imaging have greatly advanced the field of neuroscience by making it possible to manipulate and image neural activity in vivo with single-cell resolution, and applying such capability in a freely-behaving animal enables researchers to stimulate/control and visualize regions, individually or simultaneously, in order to study the correlation between neural circuits and animal behaviour. Specifically, researchers are able to use the methodology depicted below to investigate neural activity – in a freely behaving animal – in cortical regions.

cortical region calcium imaging and targeted optogenetics
cortical region targeted optogenetics
cortical region calcium imaging

Key Requirements

cortical circuit mapping
multi-colour stimulate any optogenetic probe
large field of view
synchronization

System Configurations

(1) Targeted Optogenetics + Calcium Imaging

implant_imaging_targetopto2_cortex

Based on Mightex’s OASIS Implant platform, this configuration provides:

1) Targeted optogenetics using Polygon400 to stimulate select cortical regions.

2) Large field of view to a cortical region (GCaMP or RCaMP) and stimulate sub-regions using Polygon400.

3) Super light-weight headmount for freely-behaving experiments.

4) Synchronization between imaging and external behavioural equipment.

5) Ability to probe superficial cortical regions and/or deep-brain regions.

Components: (1) OASIS Implant Platform, (2) Polygon400, (3) Any Light Source, (4) Light Source Controller(s),  (5) Any Third-Party Camera, (6) Imaging Fiber, & (7) Headmount.

(2) Targeted Optogenetics

implant_imaging_opto2_cortex

Based on Mightex’s OASIS Implant platform, this configuration provides:

1) Targeted optogenetics using Polygon400 to stimulate select cortical regions.

2) Large field of view to stimulate sub-regions within a cortical area.

3) Super light-weight headmount for freely-behaving experiments.

4) Synchronization between imaging and external behavioural equipment.

5) Ability to probe superficial cortical regions and/or deep-brain regions.

Components: (1) OASIS Implant Platform, (2) Any Light Source, (3) Light Source Controller(s),  (4) Any Third-Party Camera, (5) Imaging Fiber, & (6) Headmount.

(3) Calcium Imaging

implant_imaging2_cortex

Based on Mightex’s OASIS Implant platform, this configuration provides:

1) Large field of view to image a cortical region of a head-fixed animal (GCaMP or RCaMP).

2) Super light-weight headmount for freely-behaving experiments.

3) Synchronization between imaging and external behavioural equipment.

4) Ability to probe superficial cortical regions and/or deep-brain regions.

Components: (1) OASIS Implant Platform, (2) Any Light Source, (3) Light Source Controller(s),  (4) Any Third-Party Camera, (5) Imaging Fiber, & (6) Headmount.

Key Components

(For details, please click the icons below)

oasis implant freely-behaving calcium imaging and optogenetics
polygon400 targeted optogenetics

Additional Components

light sources
light source controllers
camera
imaging fiber
Inquiry

Calcium Imaging and Optogenetics Inquiry

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