In Vivo Neural Circuit Mapping
Optogenetics and Calcium Imaging:
Freely-Behaving
Overview

Optogenetics and calcium imaging have greatly advanced the field of neuroscience by making it possible to manipulate and image neural activity in vivo with cellular-resolution. Using these techniques in a freely-behaving animal enables researchers to stimulate/control and visualize neurons or regions, individually or simultaneously, in order to study the relationship between neural circuits and animal behaviour. Specifically, researchers are able to use the methodology depicted below to investigate neural activity – in a freely behaving animal – in both cortical regions and in the deep brain.

freely-behaving_ca2imagingtargetopto_small
calcium imaging and widefield optogenetics
calcium imaging
cortex or deep-brain

(For more details, click “Deep-Brain” or “Cortex” tabs above)

Deep-Brain

Optogenetics and calcium imaging have greatly advanced the field of neuroscience by making it possible to manipulate and image neural activity in vivo with cellular-resolution. Using these techniques in a freely-behaving animal enables researchers to stimulate/control and visualize neurons or regions, individually or simultaneously, in order to study the relationship between neural circuits and animal behaviour. Specifically, researchers are able to use the methodology depicted below to investigate neural activity – in a freely behaving animal – in the deep brain.

freely-behaving_ca2imagingtargetopto_small
calcium imaging and widefield optogenetics
calcium imaging

Key Requirements

single-cell-res_small2
multi-colour stimulate any optogenetic probe
light-weight reduced stress and longer experiments
synchronization

System Configurations

(1) Cellular-Resolution Optogenetics + Calcium Imaging

implant_imaging_targetopto3_mouse

Based on Mightex’s OASIS Implant platform, this configuration provides:

1) Cellular-resolution calcium imaging (GCaMP or RCaMP) in a freely-behaving animal.

2) Cellular-resolution optogenetics (ChR2, Chrimson, NpHr etc.) in a freely-behaving animal using Polygon400.

3) Super light-weight headmount for freely-behaving experiments.

4) Synchronization between imaging and external behavioural equipment.

5) Ability to probe superficial cortical regions and/or deep-brain regions.

Components: (1) OASIS Implant Platform, (2) Polygon400, (3) Any Light Source, (4) Light Source Controller(s),  (5) Any Third-Party Camera, (6) Imaging Fiber, & (7) Headmount + GRIN Lens.

(2) Widefield Optogenetics + Calcium Imaging

implant_imaging_opto3_mouse

Based on Mightex’s OASIS Implant platform, this configuration provides:

1) Cellular-resolution calcium imaging (GCaMP or RCaMP) in a freely-behaving animal.

2) Widefield optogenetics (ChR2, Chrimson, NpHr etc.) in a freely-behaving animal.

3) Super light-weight headmount for freely-behaving experiments.

4) Synchronization between imaging and external behavioural equipment.

5) Ability to probe superficial cortical regions and/or deep-brain regions.

Components: (1) OASIS Implant Platform, (2) Any Light Source, (3) Light Source Controller(s),  (4) Any Third-Party Camera, (5) Imaging Fiber, & (6) Headmount + GRIN Lens.

(3) Calcium Imaging

implant_imaging3_mouse

Based on Mightex’s OASIS Implant platform, this configuration provides:

1) Cellular-resolution calcium imaging (GCaMP or RCaMP) in a freely-behaving animal.

2) Super light-weight headmount for freely-behaving experiments.

3) Synchronization between imaging and external behavioural equipment.

4) Ability to probe superficial cortical regions and/or deep-brain regions.

Components: (1) OASIS Implant Platform, (2) Any Light Source, (3) Light Source Controller(s),  (4) Any Third-Party Camera, (5) Imaging Fiber, & (6) Headmount + GRIN Lens.

Key Components

(For details, please click on the icons below)

implant_small
polygon400_small_opto

Additional Components

light sources
light source controllers
camera
imaging fiber
Cortex

Optogenetics and calcium imaging have greatly advanced the field of neuroscience by making it possible to manipulate and image neural activity in vivo with cellular-resolution. Using these techniques in a freely-behaving animal enables researchers to stimulate/control and visualize neurons or regions, individually or simultaneously, in order to study the relationship between neural circuits and animal behaviour. Specifically, researchers are able to use the methodology depicted below to investigate neural activity – in a freely behaving animal – in cortical regions.

cortical region calcium imaging and targeted optogenetics
cortical region targeted optogenetics
cortical region calcium imaging

Key Requirements

cortical circuit mapping
multi-colour stimulate any optogenetic probe
large field of view
synchronization

System Configurations

(1) Cellular-Resolution Optogenetics + Calcium Imaging

implant_imaging_targetopto3_cortex_mouse

Based on Mightex’s OASIS Implant platform, this configuration provides:

1) Cellular-Resolution optogenetics using Polygon400 to stimulate select cortical regions.

2) Large field of view to a cortical region (GCaMP or RCaMP) and stimulate sub-regions using Polygon400.

3) Super light-weight headmount for freely-behaving experiments.

4) Synchronization between imaging and external behavioural equipment.

5) Ability to probe superficial cortical regions and/or deep-brain regions.

Components: (1) OASIS Implant Platform, (2) Polygon400, (3) Any Light Source, (4) Light Source Controller(s),  (5) Any Third-Party Camera, (6) Imaging Fiber, & (7) Headmount.

(2) Cellular-Resolution Optogenetics

implant_imaging_opto3_cortex_mouse

Based on Mightex’s OASIS Implant platform, this configuration provides:

1) Cellular-Resolution optogenetics using Polygon400 to stimulate select cortical regions.

2) Large field of view to stimulate sub-regions within a cortical area.

3) Super light-weight headmount for freely-behaving experiments.

4) Synchronization between imaging and external behavioural equipment.

5) Ability to probe superficial cortical regions and/or deep-brain regions.

Components: (1) OASIS Implant Platform, (2) Any Light Source, (3) Light Source Controller(s),  (4) Any Third-Party Camera, (5) Imaging Fiber, & (6) Headmount.

(3) Calcium Imaging

implant_imaging3_cortex_mouse

Based on Mightex’s OASIS Implant platform, this configuration provides:

1) Large field of view to image a cortical region of a head-fixed animal (GCaMP or RCaMP).

2) Super light-weight headmount for freely-behaving experiments.

3) Synchronization between imaging and external behavioural equipment.

4) Ability to probe superficial cortical regions and/or deep-brain regions.

Components: (1) OASIS Implant Platform, (2) Any Light Source, (3) Light Source Controller(s),  (4) Any Third-Party Camera, (5) Imaging Fiber, & (6) Headmount.

Key Components

(For details, please click the icons below)

implant_small
polygon400_small_opto

Additional Components

light sources
light source controllers
camera
imaging fiber
Examples

Freely-Behaving Targeted Optogenetics (Cortex)

This video shows an OASIS Implant system equipped with Mightex’s laser Polygon400, used to investigate the behavior of a freely-bahaving mouse. The system is capable of delivering precision targeted optogenetic stimulation to different parts of the cortex, and such stimulation in turn elicits different behaviours (Courtesy of Dr. Zhigang He and Noaf Alwahab, Harvard Medical School).

Freely-Behaving Calcium Imaging (Deep-Brain)

GCaMP imaging in the striatum of a mouse with a 600um field of view using the OASIS Implant.

Calcium imaging in a freely-behaving mouse with the OASIS Implant.

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