Polygon | Adaptors for Microscopes & Complex Systems

POLYGON INTEGRATION

The Polygon is designed for seamless integration with a wide range of commercial microscopes, including upright and inverted systems from Nikon, Olympus, Zeiss, and Leica. Dedicated microscope adaptors couple the Polygon directly into the microscope’s infinity light path, enabling precise patterned illumination while maintaining compatibility with existing imaging configurations.

Mightex offers a growing selection of microscope-specific adaptors to simplify integration across different manufacturers and microscope architectures. This flexibility allows researchers to incorporate patterned illumination into both new and existing experimental setups, expanding system capabilities as research requirements evolve.

Upright Configuration

Polygon Adaptors for Microscopes Upright

The adaptor is inserted in between the trinocular head and epi-illuminator of an upright microscopes. A 45 degree mirror is mounted in the adaptor to guide the patterned illumination towards the objective.

Inverted Configuration

Polygon Adaptors for Microscopes Inverted

The Polygon integrates with inverted microscopes by replacing the existing epi-illuminator and coupling directly to the rear illuminator port using a dedicated ring adaptor.

Custom Solutions & Unique Configurations

Beyond standard upright and inverted microscope configurations, the Polygon can be integrated into complex and custom-built imaging systems to meet specialized experimental requirements. Mightex’s engineering team works with researchers to develop tailored optical integration solutions for a wide range of complex and custom configurations, including multi-photon systems, confocal microscopes, custom imaging platforms, and beyond.

Custom & Complex Solutions

LAPP Configuration

Substage Mount

C-Mount Configuration

UPRIGHT MICROSCOPE ADAPTORS

For upright microscopes, the Polygon is typically coupled into the microscope infinity path using a cube adaptor and beam splitter. Mightex offers both single-position and 3-position adaptor configurations, allowing users to select the level of optical flexibility required for their experimental setup. Both adaptor options feature an integrated 2D tilt stage, with fine-pitch pitch and yaw adjustments for precise alignment of the Polygon projection within the microscope field of view.

3-Position Adaptor

Holds up to three mirrors or dichroics
Allows users to quickly switch between filters by sliding the desired position into the optical path
Provides greater flexibility for experiments requiring multiple optical configurations
Allows one position to be left empty to bypass the Polygon optical path without physically removing or disconnecting the Polygon
Enables rapid switching between Polygon illumination and imaging-only configurations using the empty position

INVERTED MICROSCOPE ADAPTORS

For inverted microscopes, the Polygon is typically integrated by replacing the existing epi-illuminator and coupling directly to the rear illuminator port using a dedicated ring adaptor. Ring adaptors are available with or without tilt adjustment, providing additional flexibility for optical alignment when required. The microscope’s existing filters and dichroics are then used to direct the Polygon illumination toward the sample. Mightex’s engineering team can also develop custom integration solutions and continually expands microscope compatibility to meet evolving research needs.

Adjustable Mounts

Non-Adjustable Mounts

INFINITY PORT EXPANDERS (IPX)

The IPX provides a flexible, scalable solution for all-optical imaging techniques that require multiple optical inputs. As research needs evolve and experimental setups become more complex, the IPX allows additional illumination, stimulation, imaging, and detection devices to be integrated into a single microscope infinity path without requiring a complete system redesign. Its compatibility with patterned and widefield illuminators, cameras, and laser scanners enables researchers to expand system capabilities over time while maintaining precise optical alignment and experimental flexibility.

The IPX accepts any infinity-path compatible devices;

Compatible with all Mightex Polygon models
Compatible with Mightex and 3rd party widefield epi-fluorescent illumination sources
Also supports cameras and laser scanners via appropriate adaptors

All IPX ports, excluding Port 1, feature pitch-yaw adjustable dichroic holders for precise FOV centering
Standard-size dichroic housed in an interchangeable filter cube, with optional excitation filters available for integration into the cube

Infinity Port Expander 2 (IPX2)

Expands an infinity-path port on a microscope into 2 ports, with the option to add a third

Infinity Port Expander 4 (IPX4)

Expands an infinity-path port on a microscope into a maximum of 4 ports.

INTEGRATION INTO OASIS SYSTEMS

The Polygon integrates seamlessly with the Mightex OASIS product line, extending precise spatial and temporal control of illumination to a range of in vivo imaging applications. Across OASIS Fiberscope, Macro, and Micro configurations, the Polygon can be incorporated into the optical path to add spatially patterned illumination and photostimulation while maintaining simultaneous imaging capabilities.

OASIS Fiberscope

Combines cellular-resolution imaging with patterned photostimulation in freely behaving animals
Enables precise photostimulation within deep-brain regions while simultaneously monitoring cellular activity

OASIS Macro/Micro

Supports applications ranging from large cortical FOVs with OASIS Macro to higher-magnification imaging with OASIS Micro
Provides flexible spatial and temporal control of photostimulation for advanced all-optical experiments