In Vivo Calcium Imaging
and Optogenetics:
Cortical-Circuit Mapping

Optogenetics and calcium imaging have advanced the field of neuroscience by providing precise methods for manipulating and imaging neural activity in vivo. In order to map large-scale cortical circuits in a head-fixed animal, it is essential to have the ability to perform large-scale targeted optogenetics and imaging in vivo. Below you can find application examples.

Cortex-Wide Optogenetics

This video shows our OASIS Macro and laser Polygon patterned illuminator being used to target ChR2 optogenetic stimulation (200um spots) across the cortex of an anesthetized mouse through a cranial window. This experiment was performed to elicit paw motor movements (Courtesy of University of Ottawa).

Mesoscopic Imaging and Optogenetics

with Mightex’s OASIS Macro