In Vivo Calcium Imaging
and Optogenetics:
Cortical-Circuit Mapping

Optogenetics and calcium imaging have advanced the field of neuroscience by providing precise methods for manipulating and imaging neural activity in vivo. In order to map large-scale cortical circuits in a head-fixed animal, it is essential to have the ability to perform large-scale targeted optogenetics and imaging in vivo. Below you can find application examples.

Targeted Optogenetic Stimulation of Motor and Non-Motor Cortices

Isaac Kauvar and the Deisseroth lab at Stanford used the Polygon DMD illuminator together with Mightex’s large field-of-view OASIS Macro imaging system to simultaneously optogenetically inhibit multiple regions of the mouse’s whole brain cortex. They provide evidence that cortical non-motor areas may play a causal role in motor plan execution.

A 5W 488nm laser was fiber-coupled to the Polygon after passing through Mightex’s laser mode-mixer to remove any speckles from the stimulation light. The field-of-view achieved for photostimulation was approximately 7mm in diameter. Light intensities could reach 10mW/mm^2.

Cortex-Wide Optogenetics

This video shows our OASIS Macro and laser Polygon DMD illuminator being used to target ChR2 optogenetic stimulation (200um spots) across the cortex of an anesthetized mouse through a cranial window. This experiment was performed to elicit paw motor movements (Courtesy of University of Ottawa).

Mesoscopic Imaging and Optogenetics

with Mightex’s OASIS Macro