Cortex-Wide to Single-Cell Resolution
Optogenetics and Calcium Imaging:
Cortical Circuit Mapping
Overview

Optogenetics and calcium imaging have advanced the field of neuroscience by providing precise methods for manipulating and imaging neural activity in vivo. In order to map large-scale cortical to single-cell circuits, it is essential to have the ability to perform large-scale targeted optogenetics and calcium imaging in vivo, as depicted by the methodology below.

cortex-wide optogenetics
cortical optogenetics
single-cell resolution optogenetics

(For more details, click “Cortex-Wide”, “Cortex-Region”, or “Single-Cell” tabs above)

Cortex-Wide

Optogenetics and calcium imaging have advanced the field of neuroscience by providing precise methods for manipulating and imaging neural activity in vivo. In order to map large-scale cortical circuits in a head-fixed animal, it is essential to have the ability to perform large-scale targeted optogenetics and imaging in vivo, as depicted by the methodology below.

cortex-wide calcium imaging and optogenetics
cortex-wide targeted optogenetics
calcium imaging

Key Requirements

large-scale cortical circuit mapping
multi-colour
large field of view entire cortex
synchronization

System Configurations

(1) Targeted Optogenetics + Calcium Imaging

Based on Mightex’s OASIS Macro platform, this system provides:

1) Targeted optogenetics using Polygon400 to stimulate select cortical regions.

2) Large field of view to image the entire cortex (GCaMP or RCaMP) and stimulate individual regions using Polygon400.

3) Synchronization between imaging and external behavioural/electrophysiological equipment.

4) Rotatable platform for in vivo imaging.

(2) Targeted Optogenetics

Based on Mightex’s OASIS Macro platform, this system provides:

1) Targeted optogenetics using Polygon400 to stimulate select cortical regions.

2) Large field of view to stimulate individual regions across the entire cortex.

3) Synchronization between imaging and external behavioural/electrophysiological equipment.

4) Rotatable platform for in vivo imaging.

(3) Calcium Imaging

Based on Mightex’s OASIS Macro platform, this system provides:

1) Large field of view to image the entire cortex of a head-fixed animal (GCaMP or RCaMP).

2) Synchronization between imaging and external behavioural/electrophysiological equipment.

3) Rotatable platform for in vivo imaging.

Key Components

macro_small
polygon400macro-1_small

Additional Components

light sources
light source controllers
camera
objective
Cortex-Region

Optogenetics and calcium imaging have advanced the field of neuroscience by providing precise methods for manipulating and imaging neural activity in vivo. In order to map cortical circuits in a head-fixed animal, it is essential to have the ability to perform targeted optogenetics and calcium imaging in vivo, as depicted by the methodology below.

cortical region calcium imaging and targeted optogenetics
cortical region targeted optogenetics
cortical region calcium imaging

Key Requirements

cortical circuit mapping
multi-colour
large field of view
synchronization

System Configurations

(1) Targeted Optogenetics + Calcium Imaging

Based on Mightex’s OASIS Macro platform, this system provides:

1) Targeted optogenetics using Polygon400 to stimulate select cortical regions.

2) Large field of view to a cortical region (GCaMP or RCaMP) and stimulate sub-regions using Polygon400.

3) Synchronization between imaging and external behavioural/electrophysiological equipment.

4) Rotatable platform for in vivo imaging.

(2) Targeted Optogenetics

Based on Mightex’s OASIS Macro platform, this system provides:

1) Targeted optogenetics using Polygon400 to stimulate select cortical regions.

2) Large field of view to stimulate sub-regions within a cortical area.

3) Synchronization between imaging and external behavioural/electrophysiological equipment. 

4) Rotatable platform for in vivo imaging.

(3) Calcium Imaging

Based on Mightex’s OASIS Macro platform, this system provides:

1) Large field of view to image a cortical region of a head-fixed animal (GCaMP or RCaMP).

2) Synchronization between imaging and external behavioural/electrophysiological equipment.

3) Rotatable platform for in vivo imaging.

Key Components

macro_small
polygon400macro-1_small

Additional Components

light sources
light source controllers
camera
objective
Single-Cell

Optogenetics and calcium imaging have advanced the field of neuroscience by providing precise methods for manipulating and imaging neural activity in vivo. In order to map single-cell neural circuits in the cortex head-fixed animal, it is essential to have the ability to perform cellular-resolution optogenetics and calcium imaging in vivo, as depicted by the methodology below.

freely-behaving_ca2imagingtargetopto_small
single-cell-resolutionopto_small
calcium imaging

Key Requirements

single-cell-res_small2
multi-colour
different brain regions probe cortical or deep-brain regions
synchronization

System Configurations

(1) Cellular-Resolution Optogenetics + Calcium Imaging

micro_imaging_opto1

Based on Mightex’s OASIS Micro platform, this system provides:

1) Cellular-resolution optogenetics using Polygon400 to stimulate select neurons.

2) High-resolution imaging in a cortical region (GCaMP or RCaMP) and stimulate select neurons using Polygon400.

3) Synchronization between imaging and external behavioural/electrophysiological equipment.

4) Rotatable platform for in vivo imaging.

Components: (1) OASIS Micro Platform, (2) Polygon400, (3) Any Light Source, (4) Light Source Controller(s),  (5) Any Third-Party Camera, (6) Objective.

(2) Cellular-Resolution Optogenetics

micro_opto1

Based on Mightex’s OASIS Micro platform, this system provides:

1) Cellular-resolution optogenetics using Polygon400 to stimulate select neurons.

2) High-resolution to stimulate single-cells within a cortical area.

3) Synchronization between imaging and external behavioural/electrophysiological equipment.

4) Rotatable platform for in vivo imaging. 

Components: (1) OASIS Micro Platform, (2) Polygon400, (3) Any Light Source, (4) Light Source Controller(s),  (5) Any Third-Party Camera, (6) Objective.

(3) Calcium Imaging

micro_imaging1

Based on Mightex’s OASIS Micro platform, this system provides:

1) High resolution imaging in a cortical region of a head-fixed animal (GCaMP or RCaMP).

2) Synchronization between imaging and external behavioural/electrophysiological equipment.

3) Rotatable platform for in vivo imaging.

Components: (1) OASIS Micro Platform, (2) Any Light Source, (3) Light Source Controller(s),  (4) Any Third-Party Camera, (5) Objective.

Key Components

micro_small
polygon400_small_opto

Additional Components

light sources
light source controllers
camera
objective
Examples

Cortex-Wide Targeted Optogenetics

This video shows our OASIS Macro and laser Polygon400 being used to target ChR2 optogenetic stimulation (200um spots) across the cortex of an anesthetized mouse through a cranial was performed to elicit paw motor movements (Courtesy of Dr. Greg Silasi, University of Ottawa).

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