Optogenetics and calcium imaging have advanced the field of neuroscience by providing precise methods for manipulating and imaging neural activity in vivo. In order to map large-scale cortical circuits in a head-fixed animal, it is essential to have the ability to perform large-scale targeted optogenetics and imaging in vivo. Below you can find application examples.
Isaac Kauvar and the Deisseroth lab at Stanford used the Polygon DMD illuminator together with Mightex’s large field-of-view OASIS Macro imaging system to simultaneously optogenetically inhibit multiple regions of the mouse’s whole brain cortex. They provide evidence that cortical non-motor areas may play a causal role in motor plan execution.
A 5W 488nm laser was fiber-coupled to the Polygon after passing through Mightex’s laser mode-mixer to remove any speckles from the stimulation light. The field-of-view achieved for photostimulation was approximately 7mm in diameter. Light intensities could reach 10mW/mm^2.