Optogenetics and calcium imaging have advanced the field of neuroscience by providing precise methods for manipulating and imaging neural activity in vivo. In order to map large-scale cortical circuits in a head-fixed animal, it is essential to have the ability to perform large-scale targeted optogenetics and imaging in vivo. Below you can find application examples.
This video shows our OASIS Macro and laser Polygon patterned illuminator being used to target ChR2 optogenetic stimulation (200um spots) across the cortex of an anesthetized mouse through a cranial window. This experiment was performed to elicit paw motor movements (Courtesy of University of Ottawa).