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White Paper: Patterned Illumination for Optogenetics in Cell Biology

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Optogenetic approaches in cell biology applications are becoming more and more ubiquitous as molecular toolkits expand. However, identifying which pattern illumination technologies to use with them may be challenging. This white paper provides an overview of the molecular probes available to the cell biologist and how they have been applied in different fields, going over specific examples from modulation of intracellular signaling pathways to organelle positioning dynamics.

It also compares common patterned illumination technologies that cell biologists are familiar with: galvo-based scanning and digital micromirror device (DMD) systems. It highlights how each technology applies to optogenetic approaches in cell biology emphasizing the strengths and drawbacks of their features.