With Single-Cell Resolution
Photostimulation:
Neuroscience Optogenetics
Overview

Optogenetics has advanced the field of neuroscience by providing a precise method for manipulating neuronal activity. The ability to perform single-cell resolution optogenetics enables researchers to stimulate select optogenetic-expressing neurons (e.g. ChR2, NpHr, Chrimson etc.), individually or simultaneously, in slice or culture during electrophysiological recordings or calcium imaging to study the correlation between neural activity and function, or to map neural circuitry. The methodology depicted below can be used by researchers to investigate neural activity using optogenetics in combination with electrophysiology or calcium imaging.

electrophysiology and optogenetics single-cell resolution optogenetics
calcium imaging and single-cell resolution optogenetics

Key Requirements

single-cell resolution for imaging and optogenetics
multi-colour stimulate any optogenetic probe
temporal resolution
synchronization with electrophysiology or imaging

System Configurations

(1) Electrophysiology + Optogenetics

single_upright1

Based on Mightex’s market-leading Polygon400 patterned illumination, this configuration provides:

1) Single-cell resolution optogenetics of any optogenetic probe.

2) Synchronization with electrophysiological equipment.

3) Integrates into any commercial microscope (upright or inverted).

Components: (1) Polygon400, (2) Microscope Adapter, (3) Any Light Source, (4) Light Source Controller(s), & (5) Any Third-Party Camera.

(2) Calcium Imaging + Optogenetics

single_upright1

Based on Mightex’s market-leading Polygon400 patterned illuminator, this system provides:

1) Single-cell resolution optogenetics of any optogenetic probe.

2) Synchronization with calcium imaging.

3) Integrates into any commercial microscope (upright or inverted).

Components: (1) Polygon400, (2) Microscope Adapter, (3) Any Light Source, (4) Light Source Controller(s), & (5) Any Third-Party Camera.

Main Component

polygon400 single-cell resolution optogenetics

Additional Components

microscope adapter
light sources
light source controllers
camera
Inquiry

Neuroscience Optogenetics Inquiry

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