With Cellular-Resolution
Photostimulation:
Neuroscience Optogenetics
Overview

Optogenetics has advanced the field of neuroscience by providing a precise method for manipulating neuronal activity. The ability to perform cellular-resolution optogenetics enables researchers to stimulate select optogenetic-expressing neurons (e.g. ChR2, NpHr, Chrimson etc.), individually or simultaneously, in slice or culture during electrophysiological recordings or calcium imaging to study the correlation between neural activity and function, or to map neural circuitry. The methodology depicted below can be used by researchers to investigate neural activity using optogenetics in combination with electrophysiology or calcium imaging.

electrophysiology and optogenetics single-cell resolution optogenetics
calcium imaging and single-cell resolution optogenetics

Key Requirements

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synchronization with electrophysiology or imaging

System Configurations

(1) Electrophysiology + Optogenetics

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Based on Mightex’s market-leading Polygon400 patterned illumination, this configuration provides:

1) Cellular-resolution optogenetics of any optogenetic probe.

2) Synchronization with electrophysiological equipment.

3) Integrates into any commercial microscope (upright or inverted).

Components: (1) Polygon400, (2) Microscope Adapter, (3) Any Light Source, (4) Light Source Controller(s), & (5) Any Third-Party Camera.

(2) Calcium Imaging + Optogenetics

single_upright1

Based on Mightex’s market-leading Polygon400 patterned illuminator, this system provides:

1) Cellular-resolution optogenetics of any optogenetic probe.

2) Synchronization with calcium imaging.

3) Integrates into any commercial microscope (upright or inverted).

Components: (1) Polygon400, (2) Microscope Adapter, (3) Any Light Source, (4) Light Source Controller(s), & (5) Any Third-Party Camera.

Main Component

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Additional Components

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Examples

Cellular-Resolution Optogenetics + Electrophysiology (Brain Slice)

This video shows presynaptic ChR2-expressing cells being exposed to a targeted stimulation protocol produced by the Polygon400 (right monitor) and the electrophysiological response of the recorded postsynaptic cell (left monitor)(Courtesy of Dr. Blake Richards and Matthew Tran from University of Toronto).

Cellular-resolution optogenetic stimulation of fluorescent-labeled neurons in slice using the Polygon400 (Courtesy of Dr. Blake Richards from University of Toronto).

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