In Vivo Cellular-Resolution Optogenetics and Imaging Systems
OASIS

OASIS (Optical Activation, Stimulation, and Imaging Systems) are ground-breaking platforms for all-optical cellular-resolution optogenetic stimulation and calcium imaging in vivo, both freely-behaving and head-fixed. With an OASIS, researchers can optogenetically and/or image stimulate single cells, multiple cells, and/or multiple areas simultaneously in the brain of an awake animal. The fully reconfigurable and futureproof design of the OASIS platforms makes them suitable for a wide range of in vivo applications, such as deep-brain stimulation & imaging, and cortical neural circuitry mapping etc.

Overview

Neural Circuit Mapping in Freely Behaving Animals

Cortex, Deep-Brain, and Multi-Region

calcium imaging and optogenetics freely-behaving
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Large-Scale Cortical Circuit Mapping

Investigate Neural Activity Across the Cortex

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 Cortical or Deep-Brain Circuit Mapping

With Single-Neuron or Sub-Neuron Resolution

optogenetics single-cell resolution
OASIS Micro

Key Product Features

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Target Applications

GCaMP imaging in the striatum of a mouse with a 600um field of view using the OASIS Implant.

This video shows the OASIS Implant being used in combination with the laser Polygon400 to deliver targeted optogenetic stimulation to different parts of the cortex of a freely-behaving mouse that in turn elicits different behaviours (Courtesy of Dr. Zhigang He and Noaf Alwahab, Harvard Medical School).

Calcium imaging in a freely-behaving mouse with the OASIS Implant.

This video shows our OASIS Macro and laser Polygon400 being used to target ChR2 optogenetic stimulation (200um spots) across the cortex of an anesthetized mouse through a cranial was performed to elicit paw motor movements (Courtesy of Dr. Greg Silasi, University of Ottawa).

Resources

Are you interested in all-optical methods for probing in vivo neural activity and deciding what tool is right for you?

This paper will discuss several tools that enable in vivo calcium imaging and optogenetics and their relative merits in an experimental setting. Specifically, the advantages and drawbacks of two-photon imaging, head-mounted microscopes, fiber photometry, and fiberscopes for in vivo imaging and optogenetics will be discussed. Read more.

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