In Vivo Targeted Optogenetics and Imaging Systems
OASIS

OASIS (Optical Activation, Stimulation, and Imaging Systems) are ground-breaking platforms for all-optical optogenetic stimulation and calcium imaging in vivo, both freely-behaving and head-fixed. With an OASIS, researchers can optogenetically and/or image stimulate single cells, multiple cells, and/or multiple areas simultaneously in the brain of an awake animal. The fully reconfigurable and futureproof design of the OASIS platforms makes them suitable for a wide range of in vivo applications, such as deep-brain stimulation & imaging, and cortical neural circuitry mapping etc.

Overview

Neural Circuit Mapping in Freely Behaving Animals

Cortex, Deep-Brain, and Multi-Region

calcium imaging and optogenetics freely-behaving
OASIS Implant
OASIS Macro on stereotaxic frame

Large-Scale Cortical Circuit Mapping

Investigate Neural Activity Across the Cortex

cortical circuit mapping

 Cortical or Deep-Brain Circuit Mapping

With Single-Neuron or Sub-Neuron Resolution

optogenetics single-cell resolution
OASIS Micro

Key Product Features

designed for in vivo experiments
single-cell resolution imaging and optogenetics feature
cortex or deep-brain imaging and optogenetics
reconfigurable futureproof platform feature
Polygon400 - External Equipment Synchronization
third-party camera compatible

Target Applications

GCaMP imaging in the striatum of a mouse with a 600um field of view using the OASIS Implant

This video shows the OASIS Implant being used in combination with the Polygon400 to deliver targeted optogenetic stimulation to different parts of the cortex of a freely-behaving mouse that in turn elicits different behaviours (Courtesy of Dr. Zhigang He and Noaf Alwahab, Harvard Medical School).

Calcium imaging in a freely-behaving mouse with the OASIS Implant.

GCaMP imaging in the striatum of a mouse with a 300um field of view using the OASIS Implant

Resources

Are you interested in all-optical methods for probing in vivo neural activity and deciding what tool is right for you?

This paper will discuss several tools that enable in vivo calcium imaging and optogenetics and their relative merits in an experimental setting. Specifically, the advantages and drawbacks of two-photon imaging, head-mounted microscopes, fiber photometry, and fiberscopes for in vivo imaging and optogenetics will be discussed. Read more.

White Paper Image