DESIGN, PROJECT, AND STIMULATE WITH SPEED AND PRECISION
- Illuminate multiple regions of interest (ROIs) of any shape or size
- Control the initiation, duration, and intensity of light stimulation patterns
- Target multiple individual cells or regions in your field-of-view (FOV) for sophisticated, precise circuit manipulation
- Configure grey levels to stimulate at different intensities across your FOV
- Deliver multiple wavelengths with LED and/or laser sources
- Rapidly switch between illumination wavelengths to stimulate different photoresponsive constructs
- Accepts input from a liquid lightguide, fiber-coupled laser, or both
- Wavelength range: 350nm – 1000nm
- Superior spatial resolution enables stimulation of ROIs at subcellular resolution for investigation of intracellular events and components
- User-configured, complex spatiotemporal patterns allow you to dissect the function of individual neurons within a circuit
- Resolution of 0.8μm with a x10 objective
- Store up to 2000 patterns in the onboard memory and switch patterns at speeds up to 6.6kHz
- Rapid pattern switching supports real-time adaptation and high-throughput photostimulation workflows
- Support for uploading externally-generated bitmap images that contain different grey levels
Courtesy of Raymond Dunn, PhD – Kato Lab, UC San Francisco
- Easily add the Polygon to the infinity path of any commercial or custom microscope
- Seamlessly integrate with any of our OASIS Solutions for in vitro, head-fixed, and freely-behaving experiments
- Compatible with 3rd party cameras
- 3-position adaptor accommodates different filter sets to easily switch wavelength configurations or decouple the Polygon to retain the microscope’s default imaging capabilities
- Closed-loop control automatically adjusts photostimulation based on real-time imaging feedback
- Integrate real-time pattern generation for fast, automated stimulation and imaging experiments
- Integrate compatible TTL- or TCP/IP-enabled hardware for precise synchronization and automation of photostimulation experiments
- Upload speeds as fast as 4ms/frame
WHAT IS A DMD ILLUMINATOR?
Digital Micromirror Device (DMD) arrays consist of millions of high-speed, reconfigurable micromirrors that provide spatial light modulation within microseconds. By rapidly switching individual mirrors between ON and OFF states, the DMD selectively directs light toward or away from the optical path, enabling continuous generation of dynamic digital illumination patterns from one or more light sources.
- Supports illumination patterns of virtually any shape or size, providing exceptional flexibility for complex biological samples
- Delivers precise spatial and temporal control, allowing light to be directed exactly where and when it is needed
- Adapts instantly to changing experimental requirements through software-defined pattern generation, eliminating the need for optical realignment
- Well suited for complex stimulation paradigms and high-content workflows, where multiple targets must be controlled independently and simultaneously
INTEGRATE INTO ANY COMMERCIAL SYSTEM
The Polygon is designed to integrate seamlessly into the infinity path of virtually any microscope or imaging platform. Using microscope-specific adapters, the system can be coupled to leading commercial microscope brands as well as custom imaging setups. Its flexible architecture and built-in TTL triggering capabilities enable straightforward synchronization with cameras, light sources, electrophysiology equipment, and other laboratory hardware. The Polygon also integrates with Mightex’s OASIS imaging solutions, extending patterned photostimulation capabilities to in vivo, deep-brain, and large-area cortical applications.
Upright Microscopes
Complex Imaging Systems
Compatible with custom, multi-photon, and confocal imaging platforms
Inverted Microscopes
Mightex Imaging Solutions
Compatible with the entire OASIS Solutions, providing exceptional flexibility across diverse research applications
COMPREHENSIVE SOFTWARE INTEGRATION AND CONTROL WITH POLYSCAN
The Polygon1000 is controlled through our PolyScan software, which provides comprehensive control of the Polygon1000, light sources, and cameras within a unified interface. Designed for both ease of use and advanced experimental flexibility, PolyScan offers powerful tools for calibration, pattern generation, and automated photostimulation workflows.
- Fast and intuitive calibration tools map camera pixels directly to DMD mirrors for precise pattern projection
- Built-in pattern design tools allow users to create and execute custom spatial illumination patterns and stimulation sequences
- Programmable light-source control enables the delivery of user-defined pulse trains for precise temporal stimulation
- Overlaid timestamps of triggered events for quick visualization of evoked responses
- Switch between data collection and reviewing previous sessions with one click
RECENT SUCCESS CASES
Closed-Loop Interrogation of
Whole-Brain Activity
This study combines whole-brain calcium imaging, targeted optogenetics, and custom software engineering to investigate how neural circuits implement cognitive functions such as short-term memory in the nematode C. elegans.
Raymond L. Dunn, PhD
Kato Lab, UC San Francisco
Single-Cell Isolation Using the Optoelectronic Microrobot
This research combines optoelectronic tweezer (OET)-controlled microrobots with precision cell manipulation techniques to enable the gentle isolation of sensitive cell populations that are difficult to process using conventional fluorescence-activated cell sorting (FACS) methods.
Mohamed Elsayed, PhD
Wheeler Lab, University of Toronto
Photopatterning of Multi-Domain DNA Hydrogels
This study integrates high-resolution Polygon photopatterning, multi-domain DNA hydrogels, and toehold-mediated DNA reaction networks to enable programmable DNA release at user-defined locations and times with quantitative fluorescent readout.
Misha Rubanov, PhD
Schulman Lab, Johns Hopkins University
All-Optical Interface to a 3D Neuronal Construct Mimicking Cortical Pathway Connectivity
This work leverages optogenetics, calcium imaging, and guided neuronal network architecture to emulate sensory input pathways and study information processing in three-dimensional neural constructs.
Saeed Omidi
Berdichevsky Lab, Lehigh University
OUR SUITE OF POLYGON SOLUTIONS
- Accepts a 3mm-core liquid lightguide input
- Can be used with any light source
- Wavelength range: 350nm-1000nm
- Interchangeable front tubes available for fine resolution or large FOV
- Accepts SMA-connectorized optical fiber patch cord input
- Can be used with laser light sources
- Wavelength range: 400nm-1000nm
- High-intensity or large FOV applications
- Superior spatial resolution enables stimulation of ROIs at subcellular resolution for investigation of intracellular events and components
- User-configured, complex spatiotemporal patterns allow you to dissect the function of individual neurons within a circuit
- Resolution of 0.8μm with a x10 objective
- Accepts SMA-connectorized fiber input from laser sources
- Wavelength range: 400nm-700nm
- Ultra-high contrast of 10,000,000 : 1 for highly photosensitive applications
- Greater number of grids for higher spatial resolution circuit mapping
TRUSTED BY RESEARCHERS ALL OVER THE WORLD
Publications
+170
Countries
+40
Labs
+700
SUPPORT & DEVELOPMENT
We provide comprehensive remote and in-person support throughout every stage of your experiment. From initial system installation and configuration to advanced experimental workflows, our technical and applications teams work closely with you to ensure reliable performance and smooth integration into your lab environment. Support is available for troubleshooting, optimization, and experimental planning, with responsive follow-ups to keep your research moving forward. Leveraging our global distributor network, we ensure consistent, high-quality support for users worldwide.
Our training programs are designed to help users quickly gain confidence and proficiency with both hardware and software. We offer detailed instruction on system setup, operation, and maintenance, as well as guidance on experimental workflows and best practices. For applications that require it, we also provide training on surgical procedures and preparation techniques relevant to optical imaging and optogenetics. Training can be delivered remotely or on-site, depending on your needs, and is tailored to the experience level and research goals of your team.
We maintain a robust online resource library to support your research beyond direct technical assistance. These resources include application notes, e-books, recorded webinars, tutorials, and references to relevant peer-reviewed publications. Designed to support both new and experienced users, our materials provide practical guidance, troubleshooting tips, and real-world examples to help you refine experimental design, improve data quality, and stay informed about emerging techniques.
We provide comprehensive remote and in-person support throughout every stage of your experiment. From initial system installation and configuration to advanced experimental workflows, our technical and applications teams work closely with you to ensure reliable performance and smooth integration into your lab environment. Support is available for troubleshooting, optimization, and experimental planning, with responsive follow-ups to keep your research moving forward. Leveraging our global distributor network, we ensure consistent, high-quality support for users worldwide.
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