Simultaneous Imaging and Optogenetics
Single-Cell/ Sub-Cellular Resolution
The OASIS Micro has a reconfigurable platform to be configured for multiple imaging and optogenetic applications. Please see below for an outline of the OASIS Micro components.
The OASIS Micro can be modified to accommodate multiple light sources, depending on the imaging and optogenetic application
The OASIS Micro is compatible with any light source (LED, laser, halogen lamp etc.) via lightguide or fiber. Our Polygon400 Patterned Illuminator is compatible with the OASIS Micro to selectively illuminate/stimulate neurons of interest in vivo.
The OASIS Micro can hold standard filter sets to meet the needs of multiple imaging and stimulation applications.
The OASIS Micro can be used with different objectives (Olympus, Nikon, Zeiss, Leica) that are interchangeable. The appropriate objective will depend on the size of imaging/stimulation area.
The OASIS Micro is compatible with any C-Mount camera for large-scale cortical imaging. An external camera allows the OASIS Micro to be used with high-speed/sensitivity scientific cameras for increase imaging quality for quantitative imaging capture and analysis.
Two different stage options are available depending on the animal model and region of interest. The standard translation stage allows for adjustments in the XYZ and can be tilted. The stereotaxic stage allows the objective to be adjusted around the animal from multiple angles to properly control the location of the imaging area.
The OASIS Micro is designed to be a futureproof platform that can be reconfigured by customers to suit their current and future optogenetics and imaging research on head-fixed animals. Please see below for examples of different OASIS Micro configurations.
If your specific application is not covered by the example configurations below, please contact Mightex directly for technical support.
Simultaneous widefield imaging of GCaMP or RCaMP labeled-cells and targeted red-shifted or blue-shifted optogenetics (e.g. Chrimson, JAWS, etc.) in a small cortical area or the deep-brain of a head-fixed animal.