In Vivo Single-Cell Resolution
Optogenetics and Imaging System
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OASIS Micro
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The OASIS Micro is a ground-breaking microscope platform for all-optical optogenetics and imaging in small cortical or deep-brain areas of a head-fixed animal. Simultaneously image small cortical or deep brain regions and perform neuron-specific targeted optogenetics with the OASIS Micro. Built to meet the needs of both current and future in vivo applications, the futureproof design of the OASIS Micro enables it to be reconfigured for multiple optogenetic and imaging experiments. Truly investigate single-cell neural dynamics with advanced optogenetics and imaging.

Overview
Technology

Benefits

multi-region illumination

Simultaneous Imaging and Optogenetics

single-cell resolution

Single-Cell/ Sub-Cellular Resolution

reconfigurable

Reconfigurable

rotate

Rotational Platform

Components

The OASIS Micro has a reconfigurable platform to be configured for multiple imaging and optogenetic applications. Please see below for an outline of the OASIS Micro components.

Multi-Port Illumination

The OASIS Micro can be modified to accommodate multiple light sources, depending on the imaging and optogenetic application

Light Sources

The OASIS Micro is compatible with any light source (LED, laser, halogen lamp etc.) via lightguide or fiber. Our Polygon400 Patterned Illuminator is compatible with the OASIS Micro to selectively illuminate/stimulate neurons of interest in vivo.

Filter Sets

The OASIS Micro can hold standard filter sets to meet the needs of multiple imaging and stimulation applications.

Objective

The OASIS Micro can be used with different objectives (Olympus, Nikon, Zeiss, Leica) that are interchangeable. The appropriate objective will depend on the size of imaging/stimulation area.

External Camera

The OASIS Micro is compatible with any C-Mount camera for large-scale cortical imaging. An external camera allows the OASIS Micro to be used with high-speed/sensitivity scientific cameras for increase imaging quality for quantitative imaging capture and analysis.

Stage

Two different stage options are available depending on the animal model and region of interest. The standard translation stage allows for adjustments in the XYZ and can be tilted. The stereotaxic stage allows the objective to be adjusted around the animal from multiple angles to properly control the location of the imaging area.

Configurations

OASIS Micro Configurations

The OASIS Micro is designed to be a futureproof platform that can be reconfigured by customers to suit their current and future optogenetics and imaging research on head-fixed animals. Please see below for examples of different OASIS Micro configurations.

If your specific application is not covered by the example configurations below, please contact Mightex directly for technical support.

micro_imaging_opto1

Simultaneous widefield imaging of GCaMP or RCaMP labeled-cells and targeted red-shifted or blue-shifted optogenetics (e.g. Chrimson, JAWS, etc.) in a small cortical area or the deep-brain of a head-fixed animal.

Components: (1) OASIS Micro Platform, (2) Polygon400, (3) Any Light Source, (4) Light Source Controller(s),  (5) Any Third-Party Camera, (6) Objective.

micro_opto1

Targeted red-shifted or blue-shifted optogenetics (e.g. Chrimson, JAWS, etc.) in a small cortical area or the deep-brain of a head-fixed animal.

Components: (1) OASIS Micro Platform, (2) Polygon400, (3) Any Light Source, (4) Light Source Controller(s),  (5) Any Third-Party Camera, (6) Objective.

micro_imaging1

Widefield imaging of GCaMP or RCaMP labeled-cells in a small cortical area or the deep-brain of a head-fixed animal.

Components: (1) OASIS Micro Platform, (2) Any Light Source, (3) Light Source Controller(s),  (4) Any Third-Party Camera, (5) Objective.

Resources

This application note reports on how the Polygon400 was used on the OASIS Micro for targeted optogenetic stimulation to decode olfactory codes in head-fixed mice. Read more.

OASIS Micro
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