CRACM, or channelrhodopsin-2(ChR2)-assisted circuit mapping, is an optical method used to dissect large, complex neural circuits with optogenetics (Petreanu et al. 2007). To start, ChR2 is [...]
Fiber photometry enables researchers to visualize the activity of neuron populations in a freely-behaving animal using calcium imaging (Kim et al. 2016). By ‘reading out’ neural activity, fiber [...]
Calcium imaging enables neuroscientists to visualize the activity of large neuronal populations using fluorescent activity indicators (Grienberger & Konnerth, 2012). The invention of imaging [...]
Fiber photometry has become a very popular method for in vivo calcium imaging due to its simple data output and system design. You are probably asking, what components are needed to perform fiber [...]
The invention of optogenetics has enabled scientists to use light to turn cell-type specific neural activity on or off with millisecond-precision to probe neural circuit function (Yizhar et al. [...]
If someone were to describe voltage imaging to you, it might sound quite simple. When you dig deeper, you start to appreciate the complexity associated with the biology and equipment to perform [...]
Electrophysiology is the golden standard for recording individual neuron activity. By recording electrical changes across the cell membrane, electrophysiology is able to detect action potentials [...]
Intrinsic optical imaging has its strengths and weaknesses, like many techniques. Many researchers employ this technique because it is non-invasive, requiring very little surgical intervention [...]
Intrinsic optical imaging (or intrinsic signal imaging) is a simplistic imaging method that enables scientists to indirectly record neural activity in large cortical regions non-invasively by [...]
Current advanced imaging techniques, such as calcium imaging, can record cortical activity with high spatial precision and cell-type specificity using the expression of fluorescent sensors and [...]
