With Cellular-Resolution
Optogenetics and Calcium Imaging:
Multi-Region
Overview

Optogenetics and calcium imaging have advanced the field of neuroscience by making it possible to manipulate and image single-cell neural activity in vivo. The ability to perform cellular-resolution optogenetics and imaging in multiple brain regions (surface and/or deep-brain) enables researchers to stimulate and visualize neurons, individually or simultaneously, to study the link between neural activity in multiple brain regions. Using the methodology depicted below, researchers are able to investigate neural activity in multiple regions in the cortex and/or in the deep-brain.

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calcium imaging and widefield optogenetics multi-region
multi-region calcium imaging
cortex or deep-brain

Key Requirements

multi-region probe cortical and/or deep-brain regions
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multi-colour
synchronization

System Configurations

(1) Cellular-Resolution Optogenetics + Calcium Imaging

Multi-Region

Based on Mightex’s OASIS Implant platform, this system provides:

1) Multi-region cellular-resolution calcium imaging (GCaMP or RCaMP) in a freely-behaving animal.

2) Multi-region cellular-resolution optogenetics (ChR2, Chrimson, NpHr etc.) in a freely-behaving animal using Polygon400.

3) Synchronization between imaging and external behavioural equipment.

4) Ability to probe superficial cortical regions and/or deep-brain regions.

Components: (1) OASIS Implant Platform, (2) Polygon400, (3) Any Light Source, (4) Light Source Controller(s),  (5) Any Third-Party Camera, (6) Imaging Fiber, & (7) Headmount + GRIN Lens.

(2) Widefield Optogenetics + Calcium Imaging

Multi-Region

Based on Mightex’s OASIS Implant platform, this system provides:

1) Multi-region cellular-resolution calcium imaging (GCaMP or RCaMP) in a freely-behaving animal.

2) Multi-region widefield optogenetics (ChR2, Chrimson, NpHr etc.) in a freely-behaving animal.

3) Synchronization between imaging and external behavioural equipment.

4) Ability to probe superficial cortical regions and/or deep-brain regions.

Components: (1) OASIS Implant Platform, (2) Any Light Source, (3) Light Source Controller(s),  (4) Any Third-Party Camera, (5) Imaging Fiber, & (6) Headmount + GRIN Lens.A

(3) Calcium Imaging

Multi-Region

Based on Mightex’s OASIS Implant platform, this system provides:

1) Cellular-resolution calcium imaging (GCaMP or RCaMP) in a freely-behaving animal in multiple brain regions.

2) Synchronization between imaging and external behavioural equipment.

3) Ability to probe superficial cortical regions and/or deep-brain regions.

Components: (1) OASIS Implant Platform, (2) Any Light Source, (3) Light Source Controller(s),  (4) Any Third-Party Camera, (5) Imaging Fiber, & (6) Headmount + GRIN Lens.

Key Components

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polygon400_small_opto

Additional Components

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light-sources-controllers_small
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