With Single-Cell Resolution
Optogenetics and Calcium Imaging:
Multi-Region
Overview

Optogenetics and calcium imaging have advanced the field of neuroscience by making it possible to manipulate and image single-cell neural activity in vivo. The ability to perform single-cell resolution optogenetics and imaging in multiple brain regions (surface and/or deep-brain) enables researchers to stimulate and visualize neurons, individually or simultaneously, to study the link between neural activity in multiple brain regions. Using the methodology depicted below, researchers are able to investigate neural activity in multiple regions in the cortex and/or in the deep-brain.

multi-region calcium imaging and single-cell resolution optogenetics
calcium imaging and widefield optogenetics multi-region
multi-region calcium imaging
cortex or deep-brain

Key Requirements

multi-region probe cortical and/or deep-brain regions
single-cell resolution
multi-colour
synchronization

System Configurations

(1) Single-Cell Resolution Optogenetics + Calcium Imaging

multi_implant_imaging_targetopto2

Based on Mightex’s OASIS Implant platform, this system provides:

1) Multi-region single-cell resolution calcium imaging (GCaMP or RCaMP) in a freely-behaving animal.

2) Multi-region single-cell resolution optogenetics (ChR2, Chrimson, NpHr etc.) in a freely-behaving animal using Polygon400.

3) Synchronization between imaging and external behavioural equipment.

4) Ability to probe superficial cortical regions and/or deep-brain regions.

Components: (1) OASIS Implant Platform, (2) Polygon400, (3) Any Light Source, (4) Light Source Controller(s),  (5) Any Third-Party Camera, (6) Imaging Fiber, & (7) Headmount + GRIN Lens.

(2) Widefield Optogenetics + Calcium Imaging

multi_implant_imaging_opto2

Based on Mightex’s OASIS Implant platform, this system provides:

1) Multi-region single-cell resolution calcium imaging (GCaMP or RCaMP) in a freely-behaving animal.

2) Multi-region widefield optogenetics (ChR2, Chrimson, NpHr etc.) in a freely-behaving animal.

3) Synchronization between imaging and external behavioural equipment.

4) Ability to probe superficial cortical regions and/or deep-brain regions.

Components: (1) OASIS Implant Platform, (2) Any Light Source, (3) Light Source Controller(s),  (4) Any Third-Party Camera, (5) Imaging Fiber, & (6) Headmount + GRIN Lens.A

(3) Calcium Imaging

multi_implant_imaging2

Based on Mightex’s OASIS Implant platform, this system provides:

1) Single-cell resolution calcium imaging (GCaMP or RCaMP) in a freely-behaving animal in multiple brain regions.

2) Synchronization between imaging and external behavioural equipment.

3) Ability to probe superficial cortical regions and/or deep-brain regions.

Components: (1) OASIS Implant Platform, (2) Any Light Source, (3) Light Source Controller(s),  (4) Any Third-Party Camera, (5) Imaging Fiber, & (6) Headmount + GRIN Lens.

Key Components

oasis implant freely-behaving calcium imaging and optogenetics
polygon400 single-cell resolution optogenetics

Additional Components

light sources
light source controllers
camera
imaging fiber
Inquiry

Calcium Imaging and Optogenetics Inquiry

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