New Pattern Illumination Design Features

• Insert Bitmap Images
  Users can add multiple bitmap images to an existing pattern sequence. Each selected bitmap image is inserted as a new pattern within the sequence, and adjusted to fit the effective working area (EWA). Users can control the placement of imported bitmaps within the pattern index by either selecting an existing pattern for the imported bitmaps to follow, or specifying an exact position in-between patterns prior to importing.
• Insert/Edit Grid Patterns
  User can insert a grid-scanning pattern sequence into rectangular ROIs. The tool delineates the area of selected ROIs into rows and columns of grid cells, defined by the user. Once defined, the user can then choose between a series of scanning sequences, defining the order of which each grid cell is scanned. When a grid sequence is generated, a group of new patterns is added to the pattern index, relaying the position and order of each grid cell in the sequence.

Enhanced Photometry Data Analysis Tools

• ROI Tracing Improvements

  The ROI Tracing Improvements include faster data acquisition, improved flexibility in graphing and trace display settings, streamlined UI for efficient control and additional timestamp logging with exportable files.

  With the new ROI Tracing tool, view fluorescence signal intensities in real-time. Monitor fluorescence signal within defined ROIs on the camera image. Simultaneously trace fluorescence signals from multiple ROIs to track data from multiple animals or multiple brain regions at the same time. Record and view multiple wavelengths simultaneously with live de-interleaving of frames (eg GCaMP and isosbestic) acquired from a single camera, based on customizable trace settings. Review data live or post-session in both graphical and tabular formats, with the option to export individual ROI Traces as .CSV files for data analysis.

• Export Timestamps
  In Review mode, retrieve timestamps as separate .CSV files from the camera, LED, Polygon, and triggers. The camera records the time of each captured image, the LED logs the time at the start of each waveform, and the Polygon tracks times of projected patterns. The trigger timestamps contains the activation times of each event trigger, such as TTL or user button click in software. The timestamps are synchronized to Mightex PolyEcho I/O control module.

New Light Source Features

• Advanced Pulse Train
  Users can create randomized pulsed waveforms, referred to as an “Advanced Pulse Train”. Advanced Pulse Train requires the user to define minimum and maximum time durations for pulse width and pulse interval. Once defined, the software uses gaussian distribution to select time intervals within the specified range, assigning random values to each segment of the waveform definition. The generated Randomized Pulse Trains are displayed as both a graphical preview and as tabular data, where time spans and output intensities can be manually edited by the user.
• Waveform Generator
  The Waveform Generator is a tool designed to refine the process of creating and editing complex waveforms during ongoing experiments. It provides a streamlined interface for configuring Pulse Trains, Advanced Pulse Trains, and Custom Waveforms once key device settings of the light source have been defined. The Waveform Generator simplifies waveform management for quick and repeatable waveform control.
• Importing / Exporting LED Waveforms
  Utilizing the Import/Export LED Waveform feature, users can bypass the labor-intensive process of manually re-creating complex waveforms. Users are able to import and export Pulse Waveforms, Advanced Pulse Trains, and Custom Waveforms to and from the user’s local drive as .CSV or .AWF files. This is particularly helpful for long term projects and tasks requiring repetition or collaboration with other users.

New Features to Set Up & Manage Experiments

• Session Wide Settings
  Users are able to customize session file content management, with options to save Polygon stimulation patterns, camera images and/or ROI traces to Session File, and export timestamp data as a separate .CSV file – Streamlining data review and analysis for photometry experiments.
• Device Manager
  The Device manager is a tool designed for users to search and monitor all devices within the hardware list. The feature provides a comprehensive overview of each device’s general attributes, respective wiring configuration, and general properties. Additionally, pro license users can use the device manager to; add new, import, export, duplicate, and delete custom devices within the local hardware list.