Execute Multi-Animal High-Throughput Experiments

• User-friendly, out-of-the-box solution for high-throughput fiber photometry experiments across multiple animals and/or regions of interest.
• Record population-level neural activity simultaneously from multiple fluorescent indicators.
• Versatile system with option to incorporate optogenetic stimulation.
• Overlaid timestamps of triggered events for quick visualization of evoked responses
• Designed to maximize data collection efficiency and reproducibility with large-scale, parallel experiments.

Synchronize Hardware with Behaviour

• Seamless coordination between imaging and behavioural events.
• Real-time data visualization overlaid with event timestamps from trigger sources.
• Integrated preprocessing, and effortless exporting to .csv format for analysis.
• Precise synchronization across cameras, stimulators, behavioral devices, and other third-party hardware.
• Event-related snippeting allows users to easily segment and analyze fluorescence signals corresponding to behavioral or stimulus-triggered time windows, providing a streamlined approach for extracting meaningful insights from complex datasets.

Versatile Imaging Capabilities

• Continuous monitoring of neural activity or neurotransmitter release with exceptional temporal precision.
• High acquisition speeds capture the dynamics of even the fastest biosensors, such as iGluSnFR, ensuring accurate representation of rapid neural events.
• Multi-wavelength functionality, including far-red excitation – record from multiple fluorescent indicators or combine imaging with optogenetic stimulation without spectral overlap.
• Mini lasers for core-specific widefield optogenetics provide independent control of each channel, allowing for highly targeted stimulation and flexible experimental design.
• Incorporate high-speed cameras including Mightex’s CMOS camera with speeds up to 200Hz.

• Multi-panel display for rapid experimental overview
• Visualize multiple fiber cores simultaneously
• De-interleaved traces for each ROI to isolate fluorescence-specific signal fluctuations
• Overlaid timestamps of triggered events for quick visualization of evoked responses
• Quick access to LED settings including photobleach and power meter modes
• Switch between data collection and reviewing previous sessions with one click

CUSTOMER EXAMPLE

Simultaneous Photometry & Optogenetic Stimulation in Freely Moving Mice

This study aimed to validate the dual-wavelength functionality of the OASIS platform for performing simultaneous fiber photometry and optogenetics through a single patch cable and optical fiber in freely moving animals. The platform used in this work was configured with two LEDs (405 and 488nm) for photometric excitation, a 638nm diode laser for optogenetic manipulation, and a CMOS camera for fluorescence signal capture. This setup enables validation of both the biosensor’s functionality during optogenetic activation and the opsin’s efficacy under biosensor monitoring.

To demonstrate this capability, wild-type mice were intracranially infused with AAV vectors encoding ChrimsonR and iGABASnFR, followed by implantation of a single optical fiber targeting the motor faculty. Behavioural experiments were conducted in an open-field arena under a repeated 60 s ON / 60 s OFF 638 nm laser paradigm, during which both photometry signals and locomotor activity were continuously recorded.

During the 638 nm ON phase, animals exhibited a robust increase in fluorescence intensity, accompanied by enhanced locomotor velocity and a corresponding reduction in immobility time (see the videos below demonstrating mouse activity with and without optogenetic stimulation, and the accompanying graph illustrating simultaneous photometry and movement data). These observations confirm the efficacy of OASIS in achieving artifact-free, dual-wavelength recording during optogenetic activation in behaving animals.

SUPPORT & DEVELOPMENT

Our support team goes above and beyond to ensure every customer achieves their full potential with our products. From the initial setup to advanced experimental workflows, we provide hands-on guidance tailored to each user’s needs. Our team frequently conducts customer visits to offer personalized training and ensure seamless system integration, while also developing custom hardware and software solutions for specialized applications. In addition to direct support, we maintain an extensive online resource hub that includes setup guides, tutorials, and practical examples, helping users troubleshoot and refine their experiments independently. We also provide assistance with analysis to help researchers get the most out of their results.

With an extensive network of international distributors, we provide responsive global support, ensuring that users around the world receive the same high level of expertise and care. Whether through rapid troubleshooting, detailed technical consultations, or proactive follow-ups, Mightex’s support team is committed to empowering researchers to make the most of their scientific tools.