VERSATILITY & SCALABILITY IN ONE SIMPLE PACKAGE
- One system can record 1,2,5,7, or up to 19 animals or regions simultaneously using branched fiber patch cords
- Configure high-throughput recording setups to generate large datasets faster and more efficiently
- Scale from single region experiments to complex, high-throughput studies without increasing system complexity
- Record up to 3 sensors and an isosbestic channel simultaneously
- Wavelengths are available for recording the latest red and far red sensors
- Switchable, interchangeable filter sets for quick reconfiguration
- Options are available for imaging wider FOVs or imaging through a cranial window
- Acquire data at hundreds of frames per second (up to 200Hz)
- Record even the smallest fluorescence fluctuations
- Compatible with high-sensitivity cameras such as Photometrics and PCO
- Independent photostimulation to each fiber core during recording
- Integrated optogenetics module and mini lasers
- User-configured, complex spatiotemporal patterns allow you to dissect the function of individual neurons within a circuit
- Visualize photometry traces and triggered events in real time with our turnkey fiber photometry data acquisition software
- User-friendly interface that combines data acquisition and visualization within a single window
- Real-time monitoring of incoming data for each wavelength and region of interest (ROI) during recording
- Streamlined software for deinterleaving fluorescence channels and generating traces
- Easy export to .csv files
- Processing pipeline minimizes manual intervention and reduces the risk of user error during data handling
- Standardized data outputs ensure seamless compatibility with external analysis tools
WHY CHOOSE OASIS PHOTOMETRY?
Execute Multi-Animal High-Throughput Experiments
- User-friendly, out-of-the-box solution for high-throughput fiber photometry experiments across multiple animals and/or regions of interest.
- Record population-level neural activity simultaneously from multiple fluorescent indicators.
- Versatile system with option to incorporate optogenetic stimulation.
- Overlaid timestamps of triggered events for quick visualization of evoked responses.
- Designed to maximize data collection efficiency and reproducibility with large-scale, parallel experiments.
Synchronize Hardware with Behaviour
- Seamless coordination between imaging and behavioural events.
- Real-time data visualization overlaid with event timestamps from trigger sources.
- Integrated preprocessing, and effortless exporting to .csv format for analysis.
- Precise synchronization across cameras, stimulators, behavioural devices, and other third-party hardware.
- Event-related snippeting allows users to easily segment and analyze fluorescence signals corresponding to behavioural or stimulus-triggered time windows, providing a streamlined approach for extracting meaningful insights from complex datasets.
Image with Versatile Capabilities
- Continuous monitoring of neural activity or neurotransmitter release with exceptional temporal precision.
- High acquisition speeds capture the dynamics of even the fastest biosensors, such as iGluSnFR, ensuring accurate representation of rapid neural events.
- Multi-wavelength functionality, including far-red excitation – record from multiple fluorescent indicators or combine imaging with optogenetic stimulation without spectral overlap.
- Mini lasers for core-specific widefield optogenetics provide independent control of each channel, allowing for highly targeted stimulation and flexible experimental design.
- Incorporate high-speed cameras including Mightex’s CMOS camera with speeds up to 200Hz.
PURPOSE BUILT MODULE FOR FIBER PHOTOMETRY
- Multi-panel display for rapid experimental overview
- Visualize multiple fiber cores simultaneously
- De-interleaved traces for each ROI to isolate fluorescence-specific signal fluctuations
- Overlaid timestamps of triggered events for quick visualization of evoked responses
- Quick access to LED settings including photobleach and power meter modes
- Switch between data collection and reviewing previous sessions with one click
RECENT SUCCESS CASE
This study aimed to validate the dual-wavelength functionality of the OASIS platform for performing simultaneous fiber photometry and optogenetics through a single patch cable and optical fiber in freely moving animals. The platform used in this work was configured with two LEDs (405 and 488nm) for photometric excitation, a 638nm diode laser for optogenetic manipulation, and a CMOS camera for fluorescence signal capture. This setup enables validation of both the biosensor’s functionality during optogenetic activation and the opsin’s efficacy under biosensor monitoring.
To demonstrate this capability, wild-type mice were intracranially infused with AAV vectors encoding ChrimsonR and iGABASnFR, followed by implantation of a single optical fiber targeting the motor faculty. Behavioural experiments were conducted in an open-field arena under a repeated 60 s ON / 60 s OFF 638 nm laser paradigm, during which both photometry signals and locomotor activity were continuously recorded.
During the 638 nm ON phase, animals exhibited a robust increase in fluorescence intensity, accompanied by enhanced locomotor velocity and a corresponding reduction in immobility time. These observations confirm the efficacy of OASIS in achieving artifact-free, dual-wavelength recording during optogenetic activation in behaving animals.
NO opto-stimulation
WITH opto-stimulation
Shan Sohail is an aspiring neuroscientist, currently pursuing his doctoral in Biomedical Engineering at Krembil Brain Institute. His interest is focused on understanding the mechanism of Deep Brain Stimulation in patients with brain disorders such as Parkinson’s disease and developing a more advanced treatment protocol.
Dr. Luka Milosevic is currently a Scientist at the Krembil Research Institute, Assistant Professor at the University of Toronto Institute of Biomedical Engineering, and an Affiliate Scientist at the KITE Research Institute and CRANIA. His research program is at the intersection of biomedical engineering and human neurophysiology, with interests in deep brain stimulation (DBS) mechanisms, synaptic plasticity, brain-computer interfaces, machine learning, and the development of novel, data-driven methods of brain stimulation.
“We have had an excellent experience working with Mightex and the OASIS system, which has proven to be a robust and flexible platform for integrating advanced optogenetic approaches within our current preclinical neuromodulation studies. Close collaboration with Catherine and the Mightex team has been an incredible asset, with consistent responsiveness, technically insights, and tremendous support of our research pursuits and goals.”
— Dr. Luka Milosevic, Assistant Professor at University of Toronto
— Shan Sohail, PhD Student at University of Toronto
OUR FIBER PHOTOMETRY TURNKEY SOLUTIONS
Basic
- Our basic, low-cost Turnkey Fiber photometry system which includes 1 recording wavelength plus isosbestic.
Multi-Colour
- Our multicolor Fiber Photometry System which includes wavelengths for up to 3 sensors plus isosbestic.
Integrated Optogenetics
- Our OASIS Photometry System integrates with Mightex mini lasers for simultaneous widefield optogenetics.
To see our full selection of IMAGING FIBERS and HEADMOUNTS, along with detailed specifications Click Here
SUPPORT & DEVELOPMENT
We provide comprehensive remote and in-person support throughout every stage of your experiment. From initial system installation and configuration to advanced experimental workflows, our technical and applications teams work closely with you to ensure reliable performance and smooth integration into your lab environment. Support is available for troubleshooting, optimization, and experimental planning, with responsive follow-ups to keep your research moving forward. Leveraging our global distributor network, we ensure consistent, high-quality support for users worldwide.
Our training programs are designed to help users quickly gain confidence and proficiency with both hardware and software. We offer detailed instruction on system setup, operation, and maintenance, as well as guidance on experimental workflows and best practices. For applications that require it, we also provide training on surgical procedures and preparation techniques relevant to optical imaging and optogenetics. Training can be delivered remotely or on-site, depending on your needs, and is tailored to the experience level and research goals of your team.
We maintain a robust online resource library to support your research beyond direct technical assistance. These resources include application notes, e-books, recorded webinars, tutorials, and references to relevant peer-reviewed publications. Designed to support both new and experienced users, our materials provide practical guidance, troubleshooting tips, and real-world examples to help you refine experimental design, improve data quality, and stay informed about emerging techniques.
We provide comprehensive remote and in-person support throughout every stage of your experiment. From initial system installation and configuration to advanced experimental workflows, our technical and applications teams work closely with you to ensure reliable performance and smooth integration into your lab environment. Support is available for troubleshooting, optimization, and experimental planning, with responsive follow-ups to keep your research moving forward. Leveraging our global distributor network, we ensure consistent, high-quality support for users worldwide.
