DESIGN, PROJECT, AND STIMULATE WITH SPEED AND PRECISION
- Illuminate multiple regions of interest (ROIs) of any shape or size
- Control the initiation, duration, and intensity of light stimulation patterns
- Target multiple individual cells or regions in your field-of-view (FOV) for sophisticated, precise circuit manipulation
- Configure grey levels to stimulate at different intensities across your FOV
- Deliver multiple wavelengths with LED and/or laser sources
- Rapidly switch between illumination wavelengths to stimulate different photoresponsive constructs
- Accepts input from a liquid lightguide, fiber-coupled laser, or both
- Wavelength range: 350nm – 1000nm
- Superior spatial resolution enables stimulation of ROIs at subcellular resolution for investigation of intracellular events and components
- User-configured, complex spatiotemporal patterns allow you to dissect the function of individual neurons within a circuit
- Resolution of 0.8μm with a x10 objective
- Store up to 2000 patterns in the onboard memory and switch patterns at speeds up to 6.6kHz
- Rapid pattern switching supports real-time adaptation and high-throughput photostimulation workflows
- Support for uploading externally-generated bitmap images that contain different grey levels
Courtesy of Raymond Dunn, PhD – Kato Lab, UC San Francisco
- Easily add the Polygon with the infinity path of any commercial or custom microscope
- Seamlessly integrate with any of our OASIS Solutions for in vitro, head-fixed, and freely-behaving experiments
- Compatible with 3rd party cameras
- 3-position adaptor accommodates different filter sets to easily switch wavelength configurations or decouple the Polygon to retain the microscope’s default imaging capabilities
- Closed-loop control automatically adjusts photostimulation based on real-time imaging feedback
- Integrate real-time pattern generation for fast, automated stimulation and imaging experiments
- Integrate compatible TTL- or TCP/IP-enabled hardware for precise synchronization and automation of photostimulation experiments
- Upload speeds as fast as 4ms/frame
WHAT IS A DMD ILLUMINATOR?
Digital Micromirror Device (DMD) arrays consist of millions of high-speed, reconfigurable micromirrors that provide spatial light modulation within microseconds. By rapidly switching individual mirrors between ON and OFF states, the DMD selectively directs light toward or away from the optical path, enabling continuous generation of dynamic digital illumination patterns from one or more light sources.
- Supports illumination patterns of virtually any shape or size, providing exceptional flexibility for complex biological samples
- Delivers precise spatial and temporal control, allowing light to be directed exactly where and when it is needed
- Adapts instantly to changing experimental requirements through software-defined pattern generation, eliminating the need for optical realignment
- Well suited for complex stimulation paradigms and high-content workflows, where multiple targets must be controlled independently and simultaneously
INTEGRATE INTO ANY COMMERCIAL SYSTEM
The Polygon is designed to integrate seamlessly into the infinity space of virtually any microscope or imaging platform. Using microscope-specific adapters, the system can be coupled to leading commercial microscope brands as well as custom imaging setups. Its flexible architecture and built-in TTL triggering capabilities enable straightforward synchronization with cameras, light sources, electrophysiology equipment, and other laboratory hardware. The Polygon also integrates with Mightex’s OASIS imaging solutions, extending patterned photostimulation capabilities to in vivo, deep-brain, and large-area cortical applications.
Upright Microscopes
Complex Imaging Systems
Compatible with custom, multi-photon, and confocal imaging platforms
Inverted Microscopes
Mightex Imaging Solutions
Compatible with the entire OASIS Solutions, providing exceptional flexibility across diverse research applications
COMPREHENSIVE SOFTWARE INTEGRATION AND CONTROL WITH POLYSCAN
The Polygon1000 is controlled through our PolyScan software, which provides comprehensive control of the Polygon1000, light sources, and cameras within a unified interface. Designed for both ease of use and advanced experimental flexibility, PolyScan offers powerful tools for calibration, pattern generation, and automated photostimulation workflows.
- Fast and intuitive calibration tools map camera pixels directly to DMD mirrors for precise pattern projection
- Built-in pattern design tools allow users to create and execute custom spatial illumination patterns and stimulation sequences
- Programmable light-source control enables the delivery of user-defined pulse trains for precise temporal stimulation
- Overlaid timestamps of triggered events for quick visualization of evoked responses
- Switch between data collection and reviewing previous sessions with one click
RECENT SUCCESS CASES
OUR SUITE OF POLYGON SOLUTIONS
- Accepts a 3mm-core liquid lightguide input
- Can be used with any light source
- Wavelength range: 350nm-1000nm
- Interchangeable front tubes available for fine resolution or large FOV
- Accepts SMA-connectorized optical fiber patch cord input
- Can be used with laser light sources
- Wavelength range: 400nm-1000nm
- High-intensity or large FOV applications
- Superior spatial resolution enables stimulation of ROIs at subcellular resolution for investigation of intracellular events and components
- User-configured, complex spatiotemporal patterns allow you to dissect the function of individual neurons within a circuit
- Resolution of 0.8μm with a x10 objective
- Accepts SMA-connectorized fiber input from laser sources
- Greater number of grids for higher spatial resolution circuit mapping
- Wavelength range: 400nm-700nm
- Transition between patterned stimulation and 2-photon imaging
