Combining Calcium Imaging and Electrophysiology to Investigate the Parameter-Dependent Effects of Temporal InterferenceStimulation (TIS) in Hippocampal Slices
Temporal interference stimulation (TIS) is a non-invasive neuromodulation technique that uses two high-frequency electric fields to create a low-frequency interference envelope deep within brain tissue, without stimulating the overlying regions the fields pass through. Because it can reach deep structures like the hippocampus without surgery, TIS is being explored as a potential non-invasive treatment for neurological disorders such as epilepsy.
This application note explores how researchers can characterize the stimulation-parameter-dependent effects of TIS, including: What changes in neural activity does TIS produce at different stimulation parameters? How can calcium imaging and electrophysiology be synchronized in the same in vitro preparation? And what equipment is needed to reliably combine optical imaging with electrical stimulation and recording in brain slices?
Read the full application note below to see how the OASIS Macro imaging system, combined with Mightex’s Polygon pattern illuminator, was used to record GCaMP fluorescence changes induced by TIS in a hippocampal slice model.
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Meet the Researcher
Javier Guerrero Ancil is a PhD researcher at the 4BRAIN Lab, Department of Head and Skin, Ghent University (Belgium), where he investigates non-invasive neuromodulation techniques for treating neurological disorders, with a particular focus on epilepsy. He holds a BSc in Biomedical Engineering from Mondragon University (Spain) and an MSc in Neurotechnology from the Polytechnic University of Madrid (UPM). His current research centers on understanding the mechanisms of action of temporal interference stimulation (TIS), combining signal generation and synchronization, in vitro and in vivo electrophysiology, and in vitro optical imaging in brain slices — with the ultimate goal of evaluating TIS as a non-invasive treatment for epilepsy.
“The OASIS Macro imaging system enabled me to record the changes in GCaMP fluorescence induced by TIS in my hippocampal slice model (in vitro). The ability to synchronize the imaging system from Mightex to my external stimulation and electrophysiology modules proved extremely valuable for the success of these experiments. The constant help and availability of their support team, specifically Michael Yitayew, was also key to ensure the experiments were designed and performed robustly.”
— Javier Guerrero Ancil, 4BRAIN Lab, Ghent University
See how the OASIS Macro system synchronizes calcium imaging with external stimulation and electrophysiology for experiments like this one.
Discover how the Polygon pattern illuminator delivers precise, targeted photostimulation for optogenetics and imaging experiments.