Simultaneous Multi-Region Uncaging
Photostimulation:
Uncaging
Overview

Caged compounds provide life scientists with precise ways to control cellular chemistry over space and time. From caged neurotransmitters in the neurobiology field to caged calcium chelators in physiology or caged oligonucleotides in cell biology, photouncaging has proven to be a substantial technique in the life scientist toolkit. Historically designed for photolysis under UV radiation (e.g., 2-Nitrobenzyl groups), caging groups are increasingly becoming more diverse and are now able to be activated by visible and near-infrared light (e.g., Coumarin-based and Heptamethine cyanine fluorophore-based groups), providing further flexibility to researchers and their experiments.

uncaging application

Key Requirements

sub-cellular resolution stimulate small regions
multiple ROIs uncage multiple regions simultaneously
UV/VIS/NIR wavelengths uncage multiple caging groups
synchronization with imaging equipment and cameras

System Configurations

Uncaging

single_upright1

This configuration based on Mightex’s Polygon400 provides:

1) High-intensity uncaging on sample of any shape, size or number of regions of interest.

2) High-intensity 405nm laser used with Mightex’s Polygon400 DL or high-power 365nm UV LED with Mightex’s Polygon400 DP.

3) Synchronization with imaging equipment and camera.

4) Integrates into any commercial microscope (upright or inverted).

Key components (besides a microscope): (1) A Polygon400, (2) A Microscope Adapter, (3) Lightguide-coupled Light Source(s), (4) Light Source Controller(s), & (5) A C-Mount Camera.

Main Component

polygon400_uncaging_small

Additional Components

microscope adapter
light sources
light source controllers
camera
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