Sub-Cellular Resolution Illumination
Photopatterning:
Cellular Microenvironment
Overview

How cells interact with their environment has been a fundamental question in cell biology. Over the years, scientists have looked to define the biochemical and biophysical properties of the extracellular matrix (ECM) that impact multiple cellular behaviors including migration, division, proliferation, and differentiation. In order to study its impact on cell life, many techniques have been developed to manipulate the different properties of the cellular microenviroment. Recently, the advent of light-induced biochemical compounds has allowed scientists to define the biochemical and structural features of cellular matrices by photo micropatterning and photolithography. Scientists can precisely define the spatial distribution of UV light to create unique cellular microenvironments without any use of masks or physical contact with the substrate. Cell biologists now have full spatial control of cell-matrix interactions.

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Key Requirements

sub-cell-res_microen
uv_light2
rois
sync

System Configurations

Cellular Microenviroment

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This configuration based on Mightex’s Polygon400 provides:

1) Projection of any shape, size or number of regions of interest.

2) High-power 365nm UV LED with Mightex’s Polygon400 DP.

3) Synchronization with imaging equipment, camera, and electronic stages.

4) Integrates into any commercial microscope (upright or inverted).

Key components (besides a microscope): (1) A Polygon400, (2) A Microscope Adapter, (3) Lightguide-coupled Light Source(s), (4) Light Source Controller(s), & (5) A C-Mount Camera.

Main Component

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Additional Components

microscope adapter
light sources
light source controllers
camera
Examples

Protein Photopatterning

This video shows a protein-protein interaction that is inhibited by spatially controlled blue light exposure using the Polygon400 (Courtesy of Dr. Torsten Wittman from UCSF).

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